1887

Abstract

SUMMARY: Eight strains of thermophilic bacteria were examined for the presence of covalently closed circular deoxyribonucleic acid molecules by caesium chloride-ethidium bromide density gradient centrifugation. Four of the eight strains tested, Bs1, AT61, AT62 and HB8 carried covalently closed circular DNA molecules. BS1 harboured two species of plasmids with molecular weights of 6-1 x 10 and 17-0 x 10 as determined by electron microscopy. HB8, AT61 and AT62 carried plasmids with molecular weights of 6-2 x 10, 6-6 x 10 and 6-6 x 10, respectively. Plasmids from AT61 and AT62 were indistinguishable in their electrophoretic patterns in agarose or acrylamide gel after digestion with various restriction endonucleases. This is the first evidence for the presence of plasmids in extremely thermophilic bacteria, though their functions are unknown.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-104-2-193
1978-02-01
2020-11-30
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-104-2-193
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error