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Four of nine stock cultures of Agrobacterium tested formed mixtures of two types of colonies, which were distinguished on plates of aniline blue medium because of the difference in their production of two exocellular polysaccharides. The ability of organisms from the white colonies of strain ifo12665 to produce large amounts of water-soluble polysaccharide was unstable; when kept on nutrient agar slants or in inorganic salt solution the bacteria mutated spontaneously to yield blue colonies producing large amounts of curdlan-type polysaccharide instead.
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