@article{mbs:/content/journal/micro/10.1099/00221287-103-2-359, author = "Walter, W. and Backer, A.", title = "Biosynthesis of Thiamin in Bacillus subtilis. Isolation of Mutants Accumulating 4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate", journal= "Microbiology", year = "1977", volume = "103", number = "2", pages = "359-366", doi = "https://doi.org/10.1099/00221287-103-2-359", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-103-2-359", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Thiamin-deficient mutants of Bacillus subtilis were characterized by their growth responses to the pyrimidine and thiazole moieties of the vitamin molecule and by cross-feeding tests. All mutants growing on the thiazole moiety and all mutants with an absolute requirement for thiamin fed all those growing on the pyrimidine moiety. No other cross-feeding effects were observed. From the culture fluid of a mutant growing on the thiazole moiety, two compounds were isolated which supported growth of mutants requiring the pyrimidine moiety. These compounds were identified by chromatographic, bioautographic and spectrophotometric procedures as 4-amino-5-hydroxymethyl-2-methylpyrimidine and its monophosphate derivative.", }