1887

Abstract

The ampicillin-resistant isolate KRE5367 which produced a TEM type -lactamase contained a 30 megadalton (Mdal) plasmid (pKRE5367) with a copy number of approximately 2 per chromosomal equivalent and a mole fraction guanine plus cytosine content of 0·39. Ampicillin resistance was transferred by cell-to-cell contact, and the transformation to ampicillin resistance of a sensitive strain with isolated pKRE5367 DNA proved that the structural gene for the TEM type -lactamase resided on the plasmid genome. It was shown by molecular hybridization that pKRE5367 probably contained the ampicillin translocation DNA segment (TnA) found on some R factors of enteric origin. The plasmid pKRE5367 had most of its polynucleotide sequences in common with other R factors recently isolated from . DNA-DNA hybridization studies indicated that pKRE5367 shared about 70 % base sequence homology with the two tetracycline-resistance R plasmids pLU 121 (31·5 Mdal) and pFR 16017 (33 Mdal) recently isolated in W. Germany. In addition, pKRE5367 had nearly 100 % of its polynucleotide sequences in common with the 30 Mdal ampicillin-resistance R plasmid RSF007, isolated in the U.S.A.; it shared 70 % base sequence homology with the 31·5 Mdal tetracycline-resistance plasmid pUB701, isolated in the U.K.; and it had 65 % of its polynucleotide sequences in common with the 38 Mdal tetracycline- and chloramphenicol-resistance R plasmid pR1234 isolated in the Netherlands. Despite the broad geographical range, the R plasmids of appeared to be closely related.

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/content/journal/micro/10.1099/00221287-103-2-277
1977-12-01
2022-01-18
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