Adding Chloramphenicol to cultures of growing exponentially on glycerol as carbon source, resulted in a heterogeneous population of abnormally large cells. After removal of the inhibitor and resuspension in fresh medium, the cells increased in size and the cell volumes became more normally distributed. Cells then underwent two synchronous divisions separated by 5 h; this is significantly shorter than the doubling times for culture extinction and total cell volume which are both about 7 h. There was an overall decrease in mean cell volume. The chloramphenicol-induced cell cycle was shorter than the first complete cell cycle in synchronous cultures prepared by size-selection.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error