1887

Abstract

subspecies was grown in batch cultures in which the dissolved oxygen tension (d.o.t.) was maintained at various constant levels. At a range of d.o.t. from 0.002 to 0.05 atm, which allowed good growth (mean generation time approximately 1.5 h), L-lactate was preferentially consumed before D-lactate. L-Lactate oxidation was accompanied by equimolar acetate production during exponential growth. A value for (g dry weight bacteria per mol L-lactate) of 54 was determined. Net acetate production stopped when started to use D-lactate after consumption of L-lactate. When a culture growing exponentially at the expense of L-lactate was shifted from a d.o.t. of 0.02 atm to a d.o.t. of 0.15 atm, growth was impaired, and L-lactate consumption and corresponding acetate production diminished. This decrease correlated with a loss of lactate dehydrogenase activity after the shift. appeared to possess cytochromes of the and -type and a carbon monoxide-binding pigment. Evidence is given that the principal site of oxygen damage is lactate dehydrogenase rather than the cytochrome chain.

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/content/journal/micro/10.1099/00221287-103-2-215
1977-12-01
2019-12-13
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-103-2-215
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