1887

Abstract

subspecies was grown in batch cultures in which the dissolved oxygen tension (d.o.t.) was maintained at various constant levels. At a range of d.o.t. from 0.002 to 0·05 atm, which allowed good growth (mean generation time approximately 1·5 h), -lactate was preferentially consumed before -lactate. -Lactate oxidation was accompanied by equimolar acetate production during exponential growth. A value for (g dry weight bacteria per mol -lactate) of 54 was determined. Net acetate production stopped when started to use -lactate after consumption of -lactate. When a culture growing exponentially at the expense of -lactate was shifted from a d.o.t. of 0·02 atm to a d.o.t. of 0·15 atm, growth was impaired, and -lactate consumption and corresponding acetate production diminished. This decrease correlated with a loss of lactate dehydrogenase activity after the shift. appeared to possess cytochromes of the and -type and a carbon monoxide-binding pigment. Evidence is given that the principal site of oxygen damage is lactate dehydrogenase rather than the cytochrome chain.

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1977-12-01
2021-10-28
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