1887

Abstract

Respiration of shows varying sensitivity to cyanide during exponential growth in a medium containing proteose peptone, glucose and yeast extract. After 20 h growth, respiration was stimulated up to 40% by 1 m-cyanide; sensitivity to cyanide then gradually increased until 90% inhibition of respiration was attained in late-exponential phase cultures. Salicyl hydroxamic acid alone never stimulated or inhibited respiration by more than 20% but, when added together with cyanide, inhibition was always 70 to 100% from 3h onward. Sensitivity to antimycin A was similar, but not identical to that shown to cyanide; when antimycin A was added together with salicyl hydroxamic acid, the inhibition was greater. Increased sensitivities to arsenite and malonate were also observed in late-exponential phase cultures. These changes in sensitivities were not associated with alterations in the growth medium since similar changes in sensitivity to inhibitors were observed during growth in conditioned medium. A rotenone-sensitive site is associated with cyanide-stimulated respiration and the results suggest that possesses a branched electron transport system.

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1977-12-01
2024-12-12
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References

  1. Bahr J. T., Bonner W. D. Jr 1973; Cyanide-insensitive respiration. II. Control of the alternative pathway. Journal of Biological Chemistry 348:3446–3450
    [Google Scholar]
  2. Edwards S. W., Lloyd D. 1977; Changes in oxygen uptake rates, enzyme activities, cytochrome amounts and adenine nucleotide pool levels during growth of Acanthamoeba castellanii in batch culture. Journal of General Microbiology 102:135–144
    [Google Scholar]
  3. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii. Biochemical Journal in the Press
    [Google Scholar]
  4. Evans D. A. 1973; Growth phase and the number of phosphorylation sites in the mitochondrial electron transport chain of Acanthamoeba castellanii. Journal of Protozoology 20:336–338
    [Google Scholar]
  5. Henry M. F., Nyns E. J. 1975; Cyanide-insensitive respiration. An alternative mitochondrial pathway. Sub-cellular Biochemistry 4:1–65
    [Google Scholar]
  6. Katz R. 1971; Growth phase and rotenone sensitivity in Torulopsis utilis: difference between exponential and stationary phase. FEBS Letters 12:153–156
    [Google Scholar]
  7. Katz R., Kilpatrick L., Chance B. 1971; Acquisition and loss of rotenone sensitivity in Torulopsis utilis. European Journal of Biochemistry 31:301–307
    [Google Scholar]
  8. Knowles C. J. 1976; Microorganisms and cyanide. Bacteriological Reviews 40:652–680
    [Google Scholar]
  9. Lambowitz A. M., Slayman C. W. 1971; Cyanide-resistant respiration in Neurospora crassa. Journal of Bacteriology 108:1087–1096
    [Google Scholar]
  10. Lloyd D. 1974 Mitochondria of Microorganisms. London and New York: Academic Press.;
    [Google Scholar]
  11. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode vessel. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  12. Lloyd D., Griffiths A. J. 1968; The isolation of mitochondria from the amoeba Hartmannella castellanii Neff. Experimental Cell Research 51:291–300
    [Google Scholar]
  13. Mitchell P. 1975; The protonmotive Q cycle: a general formulation. FEBS Letters 59:137–139
    [Google Scholar]
  14. Ohnishi T. 1972; Factors controlling the occurrence of site I phosphorylation in C. utilis mitochondria. FEBS Letters 24:305–309
    [Google Scholar]
  15. Rich P. R., Moore A. L. 1976; The involvement of the protonmotive ubiquinone cycle in the respiratory chain of higher plants and its relation to the branchpoint of the alternative pathway. FEBS Letters 59:339–343
    [Google Scholar]
  16. Schonbaum G. R., Bonner W. D. Jr Storey B. T., Bahr J. T. 1971; Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiology 47:124–128
    [Google Scholar]
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