The Taxonomy of the Brown Rot Fungi ( spp.) Related to their Extracellular Cell Wall-degrading Enzymes Free

Abstract

Patterns of extracellular enzymes secreted by the brown rot fungi and f. sp. were studied by column isoelectric focusing. Of the four enzymes assayed, --arabinofuranosidase (AF) showed the greatest differences between species. Thus had high AF activity at isoelectric point (pI) 5·1, had AF peaks at pI 3·0 and 7·0, showed activity over the pI range 6·0 to about 7·3 with distinct peaks at 6·7 and 7·2, and f. sp. had very high AF activity at pI 7·2. The pectinlyase (PL) patterns were also useful for identification: the characteristic molecular form of PL in cultures of and f. sp. was at pI 9·1, while the two other species showed single peaks of activity at about pI 8·5. However, some isolates also produced a peak at pI 8·5, although this was less active than the characteristic one at pI 9·1. The polygalacturonase and pectinesterase enzyme patterns were not useful for distinguishing between the fungi. The AF and PL enzyme patterns from known isolates were used to identify several ‘unknown’ brown rot cultures, including isolates from stone fruits in Australia and from , a new host in England.

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1977-11-01
2024-03-28
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References

  1. Barss H. P. 1923; Brown rot and related diseases of stone fruits in Oregon. Circular of the Oregon Agricultural Experiment Station 53:
    [Google Scholar]
  2. Boesewinkel H. J., Corbin J. B. 1970; A new record of brown rot Sclerotinia (Monilinia) laxa in New Zealand. Plant Disease Reporter 54:504–506
    [Google Scholar]
  3. Byrde R. J. W., Fielding A. H. 1968; Pectin methyl-trans-eliminase as the maceration factor of Sclerotinia fructigena and its significance in brown rot of apple. Journal of General Microbiology 52:287–297
    [Google Scholar]
  4. Byrde R. J. W., Willetts H. J. 1977 The Brown Rot Fungi of Fruits: Their Biology and Control Oxford: Pergamon Press;
    [Google Scholar]
  5. Jenkins P. T. 1965; Sclerotinia laxa Aderh. & Ruhl.: a cause of brown rot of stone fruits not previously recorded in Australia. Australian Journal of Agricultural Research 16:141–144
    [Google Scholar]
  6. Laborda F., Fielding A. H., Byrde R. J. W. 1973; Extra- and intra-cellular α-l-arabino-furanosidase of Sclerotinia fructigena. Journal of General Microbiology 79:321–329
    [Google Scholar]
  7. Penrose L. J., Tarran J., Wong A.-L. 1976; First record of Sclerotinia laxa Aderh. & Ruhl. in New South Wales: differentiation from S. fructicola (Wint.) Rehm by cultural characteristics and electrophoresis. Australian Journal of Agricultural Research 27:547–556
    [Google Scholar]
  8. Wormald H. 1954; The brown rot diseases of fruit trees. Ministry of Agriculture, Fisheries and Food, TechnicalBulletin No. 3. London: H.M.S.O.;
    [Google Scholar]
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