SUMMARY: Patterns of extracellular enzymes secreted by the brown rot fungi and f. sp. were studied by column isoelectric focusing. Of the four enzymes assayed, α-L-arabinofuranosidase (AF) showed the greatest differences between species. Thus had high AF activity at isoelectric point (pI) 5.1, had AF peaks at pI 3.0 and 7.0, showed activity over the pI range 6.0 to about 7.3 with distinct peaks at 6.7 and 7.2, and f. sp. had very high AF activity at pI 7.2. The pectinlyase (PL) patterns were also useful for identification: the characteristic molecular form of PL in cultures of and f. sp. was at pI 9.1, while the two other species showed single peaks of activity at about pI 8.5. However, some isolates also produced a peak at pI 8.5, although this was less active than the characteristic one at pI 9.1. The polygalacturonase and pectinesterase enzyme patterns were not useful for distinguishing between the fungi. The AF and PL enzyme patterns from known isolates were used to identify several ‘unknown’ brown rot cultures, including isolates from stone fruits in Australia and from a new host in England.


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