Transfection of the Anaerobe with Phage DNA Free

Abstract

The properties of a phage, , are described. Phenol-extracted phage DNA transfects Ca-treated cells and transfection is not limited to a particular stage in the growth cycle. An obligatory step in the transfection procedure is a 33-fold dilution in broth, allowing replication of the infected cells. Prolonged incubation of treated cells with DNA prior to dilution in broth results in a large decrease in phage titre. The application of this system to the investigation of the genetics of obligate anaerobes is discussed.

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1977-11-01
2024-03-29
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References

  1. Adams M. H. 1959 Bacteriophages. New York: Interscience Publishers.;
    [Google Scholar]
  2. Benzinger R., Kleber I. 1971; Transfection of E. coli and S. typhimurium spheroplasts: host-controlled restriction of infective bacteriophage P22 DNA. Journal of Virology 8:197–202
    [Google Scholar]
  3. Benzinger R., Kleber I., Huskey R. 1971; Transfection of E. coli spheroplasts. General facilitation of double-stranded DNA infectivity by protamine sulphate. Journal of Virology 7:646–650
    [Google Scholar]
  4. Bursztyn H., Sgaramella V., Ciferri O., Lederberg J. 1975; Transfectability of rough strains of S. typhimurium. Journal of Bacteriology 124:1630–1634
    [Google Scholar]
  5. Burt S. J., Woods D. R. 1976; R factor transfer to obligate anaerobes from Escherichia coli. Journal of General Microbiology 93:405–409
    [Google Scholar]
  6. Cato E. P., Johnson J. L. 1976; Reinstatement of species rank for B. fragilis, B. ovatus, B. dista-sonis, B. thetaiotaomicron, and B. vulgatus: designation of neotype strains for B. fragilis (Veillon & Zuber) Castellani and Chalmers and B. thetaiotaomicron (Distaso) Castellani and Chalmers. International Journal of Systematic Bacteriology 26:230–237
    [Google Scholar]
  7. Chao J., Chao L., Speyer J. F. 1974; Bacteriophage T4 head morphogenesis: host DNA enzymes affect frequency of petite forms. Journal of Molecular Biology 85:41–50
    [Google Scholar]
  8. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Non-chromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  9. Cosloy S. D., Oishi M. 1973; Genetic transformation in E. coli K12. Proceedings of the National Academy of Sciences of the United States of America 70:84–87
    [Google Scholar]
  10. Földes J., Trautner T. A. 1964; Infectious DNA from a newly isolated B. subtilis phage. Zeitschrift für Vererbungslehre 95:57–65
    [Google Scholar]
  11. Keller R., Traub N. 1974; The characterization of Bacteroides fragilis bacteriophage recovered from animal sera: observations on the nature of Bacteroides phage carrier cultures. Journal of General Virology 24:179–189
    [Google Scholar]
  12. Lindberg M., Sjöström J., Johansson T. 1972; Transformation of chromosomal and plasmid characters in S. aureus. Journal of Bacteriology 109:844–847
    [Google Scholar]
  13. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  14. Morris J. G. 1976; Oxygen and the obligate anaerobe. Journal of Applied Bacteriology 40:229–244
    [Google Scholar]
  15. Mosig G. 1963; Coordinate variation in density and recombination potential in T4 phage particles produced at different times after infection. Genetics 48:1195–1200
    [Google Scholar]
  16. Riva S., Polsinelli M. 1968; Relationship between competence for transfection and for transformation. Journal of Virology 2:587–593
    [Google Scholar]
  17. Romig W. R. 1962; Infection of B. subtilis with phenol-extracted bacteriophages. Virology 16:452–459
    [Google Scholar]
  18. Sjöström J., Lindberg M., Philipson L. 1972; Transfection of S. aureus with bacteriophage DNA. Journal of Bacteriology 109:285–291
    [Google Scholar]
  19. Taketo A. 1974; Sensitivity of E. coli to viral nucleic acid. VIII. Idiosyncrasy of Ca2+-dependent competence for DNA. Journal of Biochemistry 75:895–904
    [Google Scholar]
  20. Thomson J. A., Woods D. R. 1973; Properties of sphaeroplasts of a halotolerant Achromobacter strain and their infection with bacteriophage deoxyribonucleic acid. Journal of General Microbiology 74:71–76
    [Google Scholar]
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