1887

Abstract

SUMMARY: The properties of a phage, β are described. Phenol-extracted phage β DNA transfects Ca-treated cells and transfection is not limited to a particular stage in the growth cycle. An obligatory step in the transfection procedure is a 33-fold dilution in broth, allowing replication of the infected cells. Prolonged incubation of treated cells with DNA prior to dilution in broth results in a large decrease in phage titre. The application of this system to the investigation of the genetics of obligate anaerobes is discussed.

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/content/journal/micro/10.1099/00221287-103-1-181
1977-11-01
2020-01-26
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-103-1-181
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