SUMMARY: A mutant (strain G311) of W23, which requires abnormally large amounts of glycerol, was isolated and characterized. Glycerol consumption is high in the mutant even in the presence of glucose. With limiting glycerol, growth stops in mid-exponential phase and autolysis occurs (glycerol-deprival autolysis). During this autolysis the cell wall lytic enzyme seems to be more active. Inhibitors of protein synthesis or energy supply prevented both autolysis and increase in the activity of the wall lytic enzyme. Synthesis of glycerol-catabolizing enzymes in G311 was less sensitive to catabolite repression than in the parent strain. The results suggest that G311 has a defect in the regulation of the system and that exhaustion of glycerol causes an increase in autolysis.


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