1887

Abstract

Host Factor (HF) is a heat-stable RNA-binding protein required along with Q replicase for transcription of coliphage Q RNA. We have found that HF activity and antigenicity are conserved among certain Gram-negative bacterial species. We examined selected species within the genus for the presence of the HF polypeptide’s antigenicity and Q RNA replication function. While we were unable to detect either of these in or , the other eleven species tested contained cross-reacting material to HF. Furthermore, in six of these eleven species we were able to detect HF activity. The detection of HF structure and function allowed the examined species to be grouped into three categories which we have called ‘sets’. The results correlate well with those of previous studies on ribosomal RNA homology ( Palleroni ., 1973 ).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-102-2-263
1977-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/102/2/mic-102-2-263.html?itemId=/content/journal/micro/10.1099/00221287-102-2-263&mimeType=html&fmt=ahah

References

  1. Blumenthal T., Landers T. A., Weber K. 1972; Bacteriophage Qβreplicase contains the protein biosynthesis elongation factors EFTu and EFTs. Proceedings of the National Academy of Sciences of the United States of America 69:1313–1317
    [Google Scholar]
  2. Carmichael G. G., Weber K., Niveleau A., Wahba A. J. 1975; The Host Factor required for RNA phage Qβ RNA replication in vitro. Intracellular location, quantitation, and purification by poly(A)-cellulose column chromatography. Journal of Biological Chemistry 250:3607–3612
    [Google Scholar]
  3. Dubow M. S., Blumenthal T. 1975; Host Factor for coliphage Qβ RNA replication is present in Pseudomonas putida. Molecular and General Genetics 141:113–119
    [Google Scholar]
  4. Dubow M. S., Ryan T., Young R. A., Blumenthal T. 1977; Host Factor for coliphageQ/9RNA replication: presence in procaryotes and association with the 30S ribosomal subunit in Escherichia coli. Molecular and General Genetics 153:39–43
    [Google Scholar]
  5. Franze De Fernandez M. T., Eoyang L., August J. T. 1968; Factor fraction required for the synthesis of bacteriophage RNA. Nature, London 219:588–590
    [Google Scholar]
  6. Franze De Fernandez M. T., Hayward W. S., August J. T. 1972; Bacterial proteins required for replication of phage ribonucleic acid. Purification and properties of Host Factor I, a ribonucleic acid-binding protein. Journal of Biological Chemistry 247:824–831
    [Google Scholar]
  7. Kamen R. 1972; A new method for the purification of RNA-dependent RNA polymerase. Biochimica et biophysicaacta 262:88–100
    [Google Scholar]
  8. Kamen R., Kondo M., Roemer W., Weismann C. 1972; Reconstitution of replicase lacking subunit α with protein synthesis interference factor i. European Journal of Biochemistry 31:44–51
    [Google Scholar]
  9. Leffler S., Szer W. 1973; Messenger selection by bacterial ribosomes. Proceedings of the National Academy of Sciences of the United States of America 70:2364–2368
    [Google Scholar]
  10. Nishizuka Y., Lipmann F., Lucas-Lenard J. A. 1968; Isolation and properties of the Escherichia coli amino acid polymerizing enzyme. Methods in Enzymology 12:708–721
    [Google Scholar]
  11. Palleroni N. J., Doudoroff M. 1972; Some properties and taxonomic subdivisions of the genus Pseudomonas. Annual Review of Phytopathology 10:73–100
    [Google Scholar]
  12. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas. International Journal of Systematic Bacteriology 23:333–339
    [Google Scholar]
  13. Patel R. N., Ornston O. N. 1976; Immunological comparison of enzymes of the β-ketoadi pate pathway. Archives of Microbiology 110:27–36
    [Google Scholar]
  14. Senear A. W., Steitz J. A. 1976; Site-specific interaction of Qβ Host Factor and ribosomal protein Si with Qfi and R17 bacteriophage RNA’s. Journal of Biological Chemistry 251:1902–1912
    [Google Scholar]
  15. Shapiro L., Franze De Fernandez M. T., August J. T. 1968; Resolution of two factors required in the Qβ-RNA polymerase reaction. Nature, London 220:478–480
    [Google Scholar]
  16. Shine J., Dalgarno L. 1975; Terminal-sequence analysis of bacterial ribosomal RNA. Correlation between the 3´-terminal polypyrimidine sequence of 16S RNA and translational specificity of the ribosome. European Journal of Biochemistry 57:221–230
    [Google Scholar]
  17. Stallcup M. R., Sharrock W. J., Rabinowitz J. C. 1974; Ribosome and messenger specificity in protein synthesis by bacteria. Biochemical and Biophysical Research Communications 58:92–98
    [Google Scholar]
  18. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology 40:734–744
    [Google Scholar]
  19. Zinder N. D.editor 1975 RNA Phages. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-102-2-263
Loading
/content/journal/micro/10.1099/00221287-102-2-263
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error