1887

Abstract

Interspecific heterokaryons have been produced between auxotrophic mutants of and by polyethylene glycol induced fusion of somatic protoplasts. The heterokaryons grew very slowly producing colonies of irregular shape and, on complete medium, the parental strains were readily segregated. However, during long-term cultivation on minimal medium many of the heterokaryons gave rise to vigorously growing sectors characterized by the secretion of a brown pigment and by their stability when subcultured on complete medium. Protoplasts isolated from the heterokaryons gave rise to the same new colony type when regenerated on minimal medium, and to the new colony type plus the parental types on complete medium. The new colony type was assumed to be an interspecific ‘hybrid’ and showed normal vegetative morphology, regular colony shape and size but produced few conidia. Conidia formed early in the development of the ‘hybrid’ gave rise to ‘hybrid’ colonies. Various sizes of conidia were observed; the largest were uninucleate and the smallest were enucleate. Comparisons of conidial size, numbers of nuclei in cells and DNA content per nucleus for ‘hybrid’ and parental strains indicated that the ‘hybrid’ was diploid. Conidia from older cultures of the ‘hybrid’ were heterogeneous with ‘hybrid’, parental and recombinant colony types developing on germination. Cultivation of the ‘hybrid’ in the presence of benomyl gave segregation of parental and recombinant sectors.

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/content/journal/micro/10.1099/00221287-102-2-255
1977-10-01
2024-03-29
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References

  1. Anné J., Peberdy J. F. 1975; Condition for induced fusion of fungal protoplasts in polyethylene glycol solutions. Archives of Microbiology 105:201–205
    [Google Scholar]
  2. Anné J., Peberdy J. F. 1976; Induced fusion of fungal protoplasts following treatment with polyethylene glycol. Journal of General Microbiology 92:413–417
    [Google Scholar]
  3. Anné J., Eyssen H., De Somer P. 1976; Somatic hybridization of Penicillium roquefortii with P. chrysogenum after protoplast fusion. Nature; London: 262719–721
    [Google Scholar]
  4. Barron G. L., MacNeil B. H. 1962; A simplified procedure for demonstrating the parasexual cycle in Aspergillus nidulans. Canadian Journal of Botany 40:1321–1327
    [Google Scholar]
  5. Bostock G. J. 1970; DNA synthesis in the fission yeast Schizosaccharomyces pombe. Experimental Cell Research 60:16–26
    [Google Scholar]
  6. Carlson P. S. 1973; The use of protoplasts for genetic research. Proceedings of the National Academy of Sciences of the United States of America 70:598–602
    [Google Scholar]
  7. Clutterbuck A. J. 1969; Cell volume per nucleus in haploid and diploid strains of Aspergillus nidulans. Journal of General Microbiology 55:291–299
    [Google Scholar]
  8. Clutterbuck A. J., Roper J. A. 1966; A direct determination of nuclear distribution in hetero-karyons of Aspergillus nidulans. Genetical Research Cambridge: 7185–188
    [Google Scholar]
  9. Cocking E. C. 1972; Plant cell protoplasts-isolation and development. Annual Review of Plant Physiology 23:29–50
    [Google Scholar]
  10. Dodge B. O. 1936; Interspecific hybrids involving factors for ascus abortion in Neurospora. American Journal of Botany 23:555–561
    [Google Scholar]
  11. Ferenczy L., Kevei F., Szegedi M. 1975a; High-frequency fusion of fungal protoplasts. Experientia 31:1028–1029
    [Google Scholar]
  12. Ferenczy L., Kevei F., Szegedi M. 1975b; Increased fusion frequency of Aspergillus nidulans protoplasts. Experientia 31:50–52
    [Google Scholar]
  13. Ferenczy L., Szegedi M., Kevei F. 1977; Interspecific protoplast fusion and complementation in Aspergilli. Experientia 33:184–186
    [Google Scholar]
  14. Hastie A. C. 1970; Benlate induced instability of Aspergillus diploids. Nature; London: 226771
    [Google Scholar]
  15. Hastie A. C. 1973; Hybridization of Verticillium albo-atrum and Verticillium dahliae. Transactions of the British Mycological Society 60:511–523
    [Google Scholar]
  16. Lewis L. A. 1969; Genetic evidence for hybridization in an interspecific cross in the genus Sordaria. Journal of General Microbiology 59:359–367
    [Google Scholar]
  17. Peberdy J. F., Isaac S. 1976; An improved procedure for the isolation of protoplasts from Aspergillus nidulans. Microbios Letters 3:7–9
    [Google Scholar]
  18. Pontecorvo G., Roper J. A., Hemmons D. W., MacDonald K. D., Bufton A. W. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  19. Raper K. B., Fennell D. 1965 The genus Aspergillus. Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  20. Ruddle F. H., Creagan R. P. 1975; Parasexual approaches to the genetics of man. Annual Review of Genetics 9:407–486
    [Google Scholar]
  21. Wessels J. G. H. 1965; Morphogenesis and biochemical processes in Schizophyllum commune Fr. Wentia 13:1–113
    [Google Scholar]
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