1887

Abstract

Treatment of the dimorphic fungus with ethidium bromide resulted in the production of several mutants, cultures of which produce an increased proportion of yeast-like cells. A comparison of several mutant isolates with the wild type suggested that the yeast-like cell is responsible for the production of the polysaccharide, pullulan. Mutation was also shown to affect other metabolic patterns, e.g. the reduced accumulation of extracellular ethanol by the mutant. The cytochrome components of the respiratory chain in the wild type were examined by whole-cell spectrophotometry and compared with those of the mutants; the latter had a decreased cytochrome content.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-102-2-249
1977-10-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/102/2/mic-102-2-249.html?itemId=/content/journal/micro/10.1099/00221287-102-2-249&mimeType=html&fmt=ahah

References

  1. Azzi A., Santato M. 1971; Interaction of ethidium with the mitochondrial membrane: cooperative binding and energy-linked changes. Biochemical and Biophysical Research Communications 44:211–217
    [Google Scholar]
  2. Bartnicki-Garcia S. 1963; Symposium on the biochemical bases of morphogenesis in fungi. III. Mold-yeast dimorphism of Mucor. Bacteriological Reviews 27:293–304
    [Google Scholar]
  3. Bender H., Lehmann J., Wallenfels K. 1959; Pullulan, ein extracelluläres Glucan von Pullularia pullulans. Biochimicaet biophysica acta 36:309–316
    [Google Scholar]
  4. Boveris A., Oshino R., EveciŃska M., Chance B. 1971; Reduction of mitochondrial components by durohydroquinone. Biochimica et biophysica acta 245:1–16
    [Google Scholar]
  5. Carbonell L. M., Kanetsuna F., Gil F. 1970; Chemical morphology of glucan and chitin in the cell wall of the yeast phase of Paracoccidioides brasiliensis. Journal of Bacteriology 101:636–642
    [Google Scholar]
  6. Carolan G., Catley B. J., Kelly P. J. 1976; Ethanol production during the growth cycle of Aureobasidium pullulans. Biochemical Society Transactions 4:1021–1022
    [Google Scholar]
  7. Catley B. J. 1971; Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans. Applied Microbiology 22:650–654
    [Google Scholar]
  8. Catley B. J. 1973; The rate of elaboration of the extracellular polysaccharide, pullulan, during growth of Pullularia pullulans. Journal of General Microbiology 78:33–38
    [Google Scholar]
  9. Catley B. J., Kelly P. J. 1975; Metabolism of trehalose and pullulan during the growth cycle of Aureobasidium pullulans. Biochemical Society Transactions 3:1079–1081
    [Google Scholar]
  10. Chattaway F. W., Bishop R., Holmes M. R., Odds F. C., Barlow A. J. E. 1973; Enzyme activities associated with carbohydrate synthesis and breakdown in the yeast and mycelial forms of Candida albicans. Journal of General Microbiology 75:97–109
    [Google Scholar]
  11. Cooke W. B. 1959; An ecological life history of Aureobasidium pullulans (De Barry) Arnaud. Mycopathologica et mycologia applicata 12:1–45
    [Google Scholar]
  12. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for the determination of sugars and related substances. Analytical Chemistry 28:350–357
    [Google Scholar]
  13. Haddock B. A., Garland P. B. 1971; Effect of sulphate-limited growth on mitochondrial electron transfer and energy conservation between reduced nicotinamide-adenine dinucleotide and the cytochromes in Torulopsis utilis. Biochemical Journal 124:155–170
    [Google Scholar]
  14. Hall M. J., Kolankaya H. 1974; The physiology of mould-yeast dimorphism in the genus Mycotypha (Mucorales). Journal of General Microbiology 82:25–34
    [Google Scholar]
  15. Jones C. R., Kearns D. R. 1975; Identification of a unique ethidium bromide binding site on yeast t-RNAPhe by high resolution (300 MHz) nuclear magnetic resonance. Biochemistry 14:2660–2665
    [Google Scholar]
  16. Kellerman G. M., Biggs D. R., Linnane A. W. 1969; Biogenesis of mitochondria. XI. A comparison of the effects of growth limiting oxygen tension, intercalating agents and antibiotics on the obligate aerobe Candidaparapsilosis. Journal of Cell Biology 42:378–391
    [Google Scholar]
  17. Kelly P. J., Catley B. J. 1976; A purification of trehalase from baker’s yeast. Analytical Biochemistry 72:353–359*
    [Google Scholar]
  18. Miller G. L. 1959; Protein determination for large numbers of samples. Analytical Chemistry 31:965
    [Google Scholar]
  19. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
    [Google Scholar]
  20. Ramos S., Garcia-Acha I. 1975; A vegetative cycle of Pullularia pullulans. Transactions of the British Mycological Society 64:129–135
    [Google Scholar]
  21. Schulz B. E., Kraepelin G., Hinkelmann W. 1974; Factors affecting dimorphism in Mycotypha(Mucorales): a correlation with the fermentation/ respiration equilibrium. Journal of General Microbiology 82:1–13
    [Google Scholar]
  22. Slonimski P. P., Perrodin G., Croft J. H. 1968; Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells with respiratory deficient non-chromosomal ‘petites’. Biochemical and Biophysical Research Communications 30:232–239
    [Google Scholar]
  23. Storck R., Morrill R. C. 1971; Respiratory- deficient yeast-like mutant of Mucor. Biochemical Genetics 5:467–479
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-102-2-249
Loading
/content/journal/micro/10.1099/00221287-102-2-249
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error