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Summary: The relative rates of the glycolytic pathways were compared in vegetative mycelium and sporophore tissue of Agaricus bisporus. Simple radiorespirometry gave a C-1/C-6 ratio of 8·3 for pileus slices and 4·6 for vegetative mycelium. Glucose-6-phosphate dehydrogenase activity was greater in sporophores than in vegetative mycelium; glucosephosphate isomerase activity was higher in the mycelium. There was no significant difference between mannitol dehydrogenase activities in sporophore and mycelium. The results were consistent with a greater proportion of glucose oxidation occurring via the hexose monophosphate pathway in the sporophore than in the mycelium.
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