1887

Abstract

Mutations defining three new loci, and , were detected by their ability to overcome the phosphatase-negative phenotype of early-blocked asporogenous mutants in sporulation conditions. Synthesis of alkaline phosphatase by is subject to ‘vegetative’ and ‘sporulation’ controls. The mutations resulted in constitutive production of alkaline phosphatase and so could be altered in either the ‘vegetative’ or the ‘sporulation’ control system. The and mutations only affected alkaline phosphatase formation in sporulation conditions, and were considered to be sporulation specific. They rendered ‘sporulation’ alkaline phosphatase formation independent of all the mutations tested, and so independent of the control of the dependent sequence of locus expression; as the enzyme was not formed constitutively, it remained subject to some other sporulation control.

The and loci were placed between and on the genetic map; the locus was located close to . The three loci mapped separately from all known loci.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-102-1-69
1977-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/102/1/mic-102-1-69.html?itemId=/content/journal/micro/10.1099/00221287-102-1-69&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos C. 1960; Alkaline phosphatase formation in B. subtilis. Federation Proceedings 19:48
    [Google Scholar]
  2. Aronson A. I., Fitz-James P. 1976; Structure and morphogenesis of the bacterial spore coat. Bacteriological Reviews 40:360–402
    [Google Scholar]
  3. Balassa G. 1969; Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis. Molecular and General Genetics 104:73–103
    [Google Scholar]
  4. Brehm S. P., Staal S. P., Hoch J. A. 1973; Phenotypes of pleiotropic-negative sporulation mutants of Bacillus subtilis. Journal of Bacteriology 115:1063–1070
    [Google Scholar]
  5. Coote J. 1972; Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. Journal of General Microbiology 71:1–15
    [Google Scholar]
  6. Coote J. G., Mandelstam J. 1973; Use of constructed double mutants for determining the temporal order of expression of sporulation genes in Bacillus subtilis. Journal of Bacteriology 114:1254–1263
    [Google Scholar]
  7. Dicioccio R. A., Strauss N. 1973; Patterns of transcription in Bacillus subtilis during sporulation. Journal of Molecular Biology 77:325–336
    [Google Scholar]
  8. Dubnau D. 1970; Linkage map of Bacillus subtilis. In Handbook of Biochemistry, 2nd edn. section 1–39–1–45 Sober H. A. Edited by Cleveland, Ohio: The Chemical Rubber Co;
    [Google Scholar]
  9. Dubnau D., Goldthwaite C., Smith J., Mar-Mur J. 1967; Genetic mapping in Bacillus subtilis. Journal of Molecular Biology 27:163–185
    [Google Scholar]
  10. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  11. Grant W. D. 1974; Sporulation in Bacillus subtilis 168. Control of synthesis of alkaline phosphatase. Journal of General Microbiology 82:363–369
    [Google Scholar]
  12. Guespin-Michel J. F. 1971a; Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis: isolation and phenotype identification of partial revertants. Journal of Bacteriology 108:241–247
    [Google Scholar]
  13. Guespin-Michel J. F. 1971b; Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis. Genetic study of polymyxin resistant partial revertants. Molecular and General Genetics 112:243–254
    [Google Scholar]
  14. Hoch J. A. 1976; Genetics of bacterial sporulation. Advances in Genetics 18:69–99
    [Google Scholar]
  15. Hoch J. A., Nester E. W. 1973; Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:59–66
    [Google Scholar]
  16. Hranueli D., Piggot P. J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  17. Ichikawa T., Freese E. 1974; Alkaline phosphatase production in Bacillus subtilis. Biochimica et biophysica acta 338:473–479
    [Google Scholar]
  18. Iichinska E. 1960; Some physiological features of asporogenous mutants of Bacilli. Microbiology English translation of Mikrobiologiya 29:147–150
    [Google Scholar]
  19. Ito J. 1973; Pleiotropic nature of bacteriophage tolerant mutants obtained in early-blocked asporogenous mutants of Bacillus subtilis 168. Molecular and General Genetics 124:97–106
    [Google Scholar]
  20. Ito J., Mildner G., Spizizen J. 1971; Early blocked asporogenous mutants of Bacillus subtilis 168. I. Isolation and characterisation of mutants resistant to antibiotics produced by sporulatingBacillus subtilis 168. Molecular and General Genetics 112:104–109
    [Google Scholar]
  21. Janssen F. W., Lund A. J., Anderson L. E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science 127:26–27
    [Google Scholar]
  22. Lacks S., Hotchkiss R. 1960; A study of the genetic material determining an enzyme activity in Pneumococcus. Biochimica et biophysica acta 39:508–518
    [Google Scholar]
  23. Le Hégarat J. -C., Anagnostopoulos C. 1969; Localisation chromosomique d’un gène gouvernant la synthèse d’une phosphatase alcaline chez Bacillus subtilis. Comptes rendus hebdomadaire des séances de l’Académie des sciences 269:2048–2050
    [Google Scholar]
  24. Le Hégarat J. -C., Anagnostopoulos C. 1973; Purification, subunit structure and properties of two repressible phosphohydrolases of Bacillus subtilis. European Journal of Biochemistry 39:525–539
    [Google Scholar]
  25. Lepesant-Kejzlarova J., Lepesant J.-A., Walle J., Billault A., Dedonder R. 1975; Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome. Journal of Bacteriology 121:823–834
    [Google Scholar]
  26. Michel J. F., Cami B. 1969; Sélection de mutants de Bacillus subtilis bloqués au début de la sporulation - nature des mutations sélectionnées. Annales de l’Institut Pasteur 116:3–18
    [Google Scholar]
  27. Miki T., Minima Z., Ikeda Y. 1965; The genetics of alkaline phosphatase formation in Bacillus subtilis. Genetics 52:1093–1100
    [Google Scholar]
  28. Miller J. H. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Nester E. W., Schafer M., Lederberg J. 1963; Gene linkage in DNA transfer: a cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics 48:529–551
    [Google Scholar]
  30. Nukushina J. -I., Ikeda Y. 1969; Genetic analysis of the developmental processes during germination and outgrowth of Basillus subtilis spores with temperature-sensitive mutants. Genetics 63:63–74
    [Google Scholar]
  31. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  32. Piggot P. J. 1975; Characterization of a cym mutant of Bacillus subtilis. Journal of General Microbiology 89:371–374
    [Google Scholar]
  33. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  34. Ryter A. 1965; Étude morphologique de la sporulation de Bacillus subtilis. Annales de l’Institut Pasteur 108:40–60
    [Google Scholar]
  35. Sadoff H. L., Bach J. A., Kools J. W. 1965; Significance of multiple forms of glucose dehydrogenase in relationship to its heat resistance. In Spores III pp. 97–110 Cambell L. L., Halvorson H. O. Edited by Ann Arbor, Michigan: American Society for Microbiology;
    [Google Scholar]
  36. Schaeffer P., Ionesco H. 1960; Contribution à l’étude génétique de la sporogénèse bactérienne. Comptes rendus hebdomadaire des séances de l’Académie des sciences 251:3125–3127
    [Google Scholar]
  37. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Collogues internationaux du Centre national de la recherche scientifique 124:553–563
    [Google Scholar]
  38. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proceedings of the National Academy of Sciences of the United States of America 44:1072–1078
    [Google Scholar]
  39. Sterlini J., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to the development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  40. Sumida-Yasumoto C., Doi R. H. 1974; Transcription from the complementary deoxyribonucleic acid strands of Bacillus subtilis during various stages of sporulation. Journal of Bacteriology 117:775–782
    [Google Scholar]
  41. Waites W. M., Kay D., Dawes I. W., Wood D. A., Warren S. C., Mandelstam J. 1970; Sporulation in Bacillus subtilis: correlation of biochemical events with morphological changes in asporogenous mutants. Biochemical Journal 118:667–676
    [Google Scholar]
  42. Warren S. C. 1968; Sporulation in Bacillus subtilis. Biochemical changes. Biochemical Journal 109:811–818
    [Google Scholar]
  43. Wood D. A. 1972; Sporulation in Bacillus subtilis. Properties and time of synthesis of alkali-soluble protein of the spore coat. Biochemical Journal 130:505–514
    [Google Scholar]
  44. Young F. E., Wilson G. A. 1972; Genetics of Bacillus subtilis and other gram-positive sporulating bacilli. In Spores v pp. 77–106 Halvorson H. O., Hanson R., Campbell L. L. Edited by Washington, D.C: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-102-1-69
Loading
/content/journal/micro/10.1099/00221287-102-1-69
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error