1887

Abstract

synthesized up to 70% of its dry weight as poly--hydroxybutyrate when grown in batch or oxygen-limited chemostat cultures on a glucose/ammonium salts medium. In a series of steady states during transition from oxygen to ammonium limitation and at different dilution rates in the chemostat, the poly--hydroxybutyrate content of the organism decreased to a minimum of 5 to 10 % of the dry weight at an oxygen inflow rate of 1·25 % (v/v) in 400 ml argon min. At higher dissolved oxygen tensions the polymer content increased to a new maximum of 20 to 40 % of the dry weight, depending upon the dilution rate, before declining to a negligible value. In contrast, nitrogen-grown organisms displayed a steady decrease in polymer content with increasing oxygen concentration. This difference in behaviour is attributed to the greater demand for reducing power and ATP by nitrogen-fixing cultures preventing the operation of respiratory control which, it is suggested, occurs in ammonium-grown cultures over a limited range of oxygen supply rates until respiratory protection and uncoupled electron transport intervene.

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1977-09-01
2024-04-19
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References

  1. Chaney A. L., Marbach E. P. 1962; Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130–132
    [Google Scholar]
  2. Daesch G., Mortenson L. E. 1972; Effect of ammonia on the synthesis and function of the N2-fixing enzyme system in Clostridium pasteurianum. Journal of Bacteriology 110:103–109
    [Google Scholar]
  3. Dalton H., Postgate J. R. 1968; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures. Journal of General Microbiology 54:463–473
    [Google Scholar]
  4. Dalton H., Postgate J. R. 1969; Growth and physiology of Azotobacter chroococcum in continuous culture. Journal of General Microbiology 56:307–319
    [Google Scholar]
  5. Dawes E. A., Senior P. J. 1973; The role and regulation of energy reserve polymers in microorganisms. Advances in Microbial Physiology 10:135–266
    [Google Scholar]
  6. Drozd J., Postgate J. R. 1970; Effect of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. Journal of General Microbiology 63:63–73
    [Google Scholar]
  7. Drozd J. W., Tubb R. S., Postgate J. R. 1972; A chemostat study of the effect of fixed nitrogen sources on nitrogen fixation, membranes and free amino acids in Azotobacter chroococcum. Journal of General Microbiology 73:221–232
    [Google Scholar]
  8. Haaker H., Veeger C. 1976; Regulation of respiration and nitrogen fixation in different types of Azotobacter vinelandii. European Journal of Biochemistry 63:499–507
    [Google Scholar]
  9. Harrison D. E. F. 1976; The regulation of respiration rate in growing bacteria. Advances in Microbial Physiology 14:243–3I3
    [Google Scholar]
  10. Hine P. W., Lees H. 1976; The growth of nitrogen-fixing Azotobacter chroococcum in continuous culture under intense aeration. Canadian Journal of Microbiology 22:611–618
    [Google Scholar]
  11. Jackson F. A., Dawes E. A. 1976; Regulation of the tricarboxylic acid cycle and poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation. Journal of General Microbiology 97:303–312
    [Google Scholar]
  12. Jones C. W., Brice J. M., Wright V., Ackrell B. A. C. 1973; Respiratory protection of nitro- genase in Azotobacter vinelandii. FEBS Letters 29:77–81
    [Google Scholar]
  13. Kleiner D. 1975; Ammonium uptake by nitrogen fixing bacteria. I. Azotobacter vinelandii. Archives of Microbiology 104:163–169
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Mackereth F. J. H. 1964; An improved galvanic cell for determination of oxygen concentration in fluids. Journal of Scientific Instruments 41:38–41
    [Google Scholar]
  16. Nagatani H., Shimizu M., Valentine R. C. 1971; The mechanism of ammonia assimilation in nitrogen fixing bacteria. Archiv für Mikrobiologie 79:164–175
    [Google Scholar]
  17. Parker C. A. 1954; Effect of oxygen on nitrogen fixation by Azotobacter. Nature; London: 173780–781
    [Google Scholar]
  18. Phillips D. A., Johnson M. J. 1961; Aeration in fermentations. Journal of Biochemical and Microbiological Technology and Engineering 3:277–309
    [Google Scholar]
  19. Postgate J. R., Crumpton J., Hunter J. R. 1961; The measurement of bacterial viabilities by slide culture. Journal of General Microbiology 24:15–24
    [Google Scholar]
  20. Senior P. J., Dawes E. A. 1971; Poly-β-hydroxybutyrate biosynthesis and the regulation of glucose metabolism in Azotobacter beijerinckii. Biochemical Journal 125:55–66
    [Google Scholar]
  21. Senior P. J., Dawes E. A. 1973; The regulation of poly-β-hydroxybutyiate metabolism in Azotobacter beijerinckii. Biochemical Journal 134:225–238
    [Google Scholar]
  22. Senior P. J., Beech G. A., Ritchie G. A. F., Dawes E. A. 1972; The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. Biochemical Journal 128:1193–1201
    [Google Scholar]
  23. Slepecky R. A., Law J. H. 1960; A rapid spectrophotometric assay of α, β-unsaturated acids and β-hydroxy acids. Analytical Chemistry 32:1697–1699
    [Google Scholar]
  24. Smith D. D., Wyss O. 1969; The rapid loss of viability of Azotobacter in aqueous solutions. Antonie van Leeuwenhoek 35:84–96
    [Google Scholar]
  25. Stockdale H., Ribbons D. W., Dawes E. A. 1968; Occurrence of poly-β-hydroxybutyrate in the Azotobacteriaceae. Journal of Bacteriology 95:1798–1803
    [Google Scholar]
  26. Trevelyan W. E., Harrison J. S. 1952; Fractionation and micro-determination of cell carbohydrates. Biochemical Journal 50:298–305
    [Google Scholar]
  27. Ward A. C., Dawes E. A. 1973; A disk assay for poly-β-hydroxybutyrate. Analytical Biochemistry 52:607–613
    [Google Scholar]
  28. Yates M. G., Jones C. W. 1974; Respiration and nitrogen fixation in Azotobacter. Advances in Microbial Physiology 11:97–135
    [Google Scholar]
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