1887

Abstract

Theoretical models of nitrification were tested using an experimental model consisting of a column of glass beads inoculated with nitrifying bacteria, through which was passed a constant flow of substrate at non-limiting concentration. Effluent was analysed for conversion products. Both zero-order and first-order rate constants for substrate conversion decreased by 1·4% for each 1 ml h decrease in flow rate presumably because of the development of oxygen limitation. Logistic growth kinetics described growth adequately and bacteria were distributed evenly throughout the column after establishment of steady states. Bacterial concentrations of 1·4 × 10 ml and conversion rates of 5·62 × 10 p.p.m. N as NO h organism were obtained for Nitrobacter and 6·47 × 10 ml and 3·75 × 10 p.p.m. N as NH h organism for Nitrosomonas. During periods of oxygen starvation a lag was observed following increases in flow rate producing undershoots in product concentrations before establishment of new steady states.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-102-1-111
1977-09-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/102/1/mic-102-1-111.html?itemId=/content/journal/micro/10.1099/00221287-102-1-111&mimeType=html&fmt=ahah

References

  1. Ardakani M. S., Rehbock J. T., Mclaren A. D. 1973; Oxidation of nitrite to nitrate in a soil column. Proceedings of the Soil Science Society of America 37:53–56
    [Google Scholar]
  2. Ardakani M. S., Rehbock J. T., Mclaren A. D. 1974; Oxidation of ammonium to nitrate in a soil column. Proceedings of the Soil Science Society of America 38:69–99
    [Google Scholar]
  3. Bazin M. J., Saunders P. T. 1973; Dynamics of nitrification in a continuous flow system. Soil Biology and Biochemistry 5:531–543
    [Google Scholar]
  4. Bazin M. J., Saunders P. T., Prosser J. I. 1976; Models of microbial interactions in the soil. CRC Critical Reviews in Microbiology 4:463–498
    [Google Scholar]
  5. Cho C. M. 1971; Convective transport of ammonium with nitrification in soil. Canadian Journal of Soil Science 51:339–350
    [Google Scholar]
  6. Van Droogenbroeck R., Laudelout H. 1967; Phosphate requirements of the nitrifying bacteria. Antonie van Leeuwenhoek 33:287–296
    [Google Scholar]
  7. Green J. R. 1970; A test for dependence. Bio- metrika 57:205–206
    [Google Scholar]
  8. Hooper A. B., Terry K. R. 1974; Photoinactivation of ammonia oxidation in Nitro- somonas. Journal of Bacteriology 119:899–906
    [Google Scholar]
  9. Mclaren A. D. 1971; Kinetics of nitrification in soil: growth of the nitrifiers. Proceedings of the Soil Science Society of America 35:91–95
    [Google Scholar]
  10. Macura J., Kunc F. 1965; Continuous flow method in soil microbiology. V. Nitrification. Folia Microbiologica 10:125–135
    [Google Scholar]
  11. Meynell G. G., Meynell E. 1970 Theory and Practice in Experimental Biology. London: Cambridge University Press;
    [Google Scholar]
  12. Pielou E. C. 1969 An Introduction to Mathematical Ecology. New York: Wiley Interscience;
    [Google Scholar]
  13. Powlson D. S., Jenkinson D. S. 1971; Inhibition of nitrification in soil by carbon disulphide from rubber bungs. Soil Biology and Biochemistry 3:267–269
    [Google Scholar]
  14. Prosser J. I., Gray T. R. G. 1977; Use of finite difference method to study a model system of nitrification at low substrate concentrations. Journal of General Microbiology 102:119–128
    [Google Scholar]
  15. Saunders P. T., Bazin M. J. 1973a; Attachment of micro-organisms in a packed column: metabolite diffusion through the microbial film as a limiting factor. Journal of Applied Chemistry and Biotechnology 23:847–853
    [Google Scholar]
  16. Saunders P. T., Bazin M. J. 1973b; Nonsteady state studies of nitrification in soil: theoretical considerations. Soil Biology and Biochemistry 5:545–557
    [Google Scholar]
  17. Skinner F. A., Walker N. 1961; Growth of Nitrosomonas europaea in batch and continuous culture. Archiv für Mikrobiologie 38:339–349
    [Google Scholar]
  18. Smith A. J., Hoare D. S. 1968; Acetate assimilation by Nitrobacter agilis in relation to its ‘obligate autotrophy’. Journal of Bacteriology 95:844–855
    [Google Scholar]
  19. Soriano S., Walker N. 1968; Isolation of ammonia-oxidising autotrophic bacteria. Journal of Applied Bacteriology 31:493–497
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-102-1-111
Loading
/content/journal/micro/10.1099/00221287-102-1-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error