1887

Abstract

Several enzymes have been assayed in grown on different substrates. The enzymes which are involved in growth on C compounds were induced by methanol and not repressed by succinate. When succinate-grown bacteria were resuspended in medium containing methanol, four enzymes unique to growth on C compounds (hydroxypyruvate reductase, serine-glyoxylate aminotransferase, methanol dehydrogenase and glycerate kinase) were fully induced by the time growth began. When methanol-grown bacteria were resuspended in medium containing succinate, all four enzyme activities decreased. Several mutants unable to grow on C compounds were examined for deficiencies in the enzymes specific for growth on these compounds. Seven of the mutants were pleiotropic, and six were not revertible by chemical mutagens, suggesting the possibility of genetic linkage or the presence of a regulon for the genes involved in C metabolism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-101-2-327
1977-08-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/101/2/mic-101-2-327.html?itemId=/content/journal/micro/10.1099/00221287-101-2-327&mimeType=html&fmt=ahah

References

  1. Blackmore M. A., Quayle J. R. 1970; Microbial growth on oxalate by a route not involving glyoxylate carboligase. Biochemical Journal 118:53–59
    [Google Scholar]
  2. Canovas J. L., Kornberg H. L. 1969; Phosphoenolpyruvate carboxylase from Escherichia coli. Methods in Enzymology 13:288–292
    [Google Scholar]
  3. Colby J., Zatman L. J. 1972; Hexose phosphate synthase and tricarboxylic acid cycle enzymes in bacterium 4B6, an obligate methylotroph. Biochemical Journal 128:1374–1276
    [Google Scholar]
  4. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle. Biochemical Journal 72:3P
    [Google Scholar]
  5. Dunstan P. M., Anthony C., Drabble W. T. 1972; Microbial metabolism of C1 and C2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AMI on ethanol and on C1 compounds. Biochemical Journal 128:107–115
    [Google Scholar]
  6. Greenberg D. M. 1971; N5-Formyltetrahydro-folate acid cyclodehydrase. Methods in Enzymology 18B:786–789
    [Google Scholar]
  7. Heptinstall J., Quayle J. R. 1970; Pathway leading to and from serine during growth of Pseudomonas AMI on C1 compounds or succinate. Biochemical Journal 117:563–572
    [Google Scholar]
  8. Johnson P. A., Quayle J. R. 1964; Microbial growth on C1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol- grown Pseudomonas AMI . Biochemical Journal 93:281–290
    [Google Scholar]
  9. Large P. J., Carter R. H. 1973; Specific activities of enzymes of the serine pathway of carbon assimilation in Pseudomonas aminovorans and Pseudomonas ms grown on methylamine. Biochemical Society Transactions 1:1291–1293
    [Google Scholar]
  10. Large P. J., Quayle J. R. 1963; Microbial growth on C1compounds. 5. Enzyme activities in extracts of Pseudomonas AMI. Biochemical Journal 87:386–395
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. O’Connor M. L., Hanson R. S. 1975; Serine transhydroxymethylase isoenzymes from a facultative methylotroph. Journal of Bacteriology 124:985–996
    [Google Scholar]
  13. O’Connor M. L., Wopat A. E., Hanson R. S. 1977; Genetic transformation of Methylohacterium organophilum. Journal of General Microbiology 98:265–272
    [Google Scholar]
  14. Patel R. N., Mandy W. J., Hoare D. S. 1973; Physiological studies of methane- and methanol- oxidizing bacteria: immunological comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus and Pseudomonas sp. M27. Journal of Bacteriology 113:937–945
    [Google Scholar]
  15. Patt T. E., Cole G. C., Bland J., Hanson R. S. 1974; Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy. Journal of Bacteriology 120:955–964
    [Google Scholar]
  16. Patt T. E., Cole G. C., Hanson R. S. 1976; Methylobacterium, a new genus of facultatively methylotrophic bacteria. International Journal of Systematic Bacteriology 26:226–229
    [Google Scholar]
  17. Quayle J. R. 1972; The metabolism of one-carbon compounds by micro-organisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  18. Salem A. R., Wagner C., Hacking A. J., Quayle J. R. 1973; The metabolism of lactate and pyruvate by Pseudomonas AMI. Journal of General Microbiology 76:375–388
    [Google Scholar]
  19. Srere P. A., Brazil H., Gonen L. 1963; The citrate-condensing enzyme of pigeon breast muscle and moth flight muscle. Acta chemica scandinavica 17:129–134
    [Google Scholar]
  20. Tanaka N., Hanson R. S. 1975; Regulation of the tricarboxylic acid cycle in Gram-positive, facultatively anaerobic bacilli. Journal of Bacteriology 122:215–223
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-101-2-327
Loading
/content/journal/micro/10.1099/00221287-101-2-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error