Summary: The biochemical events of spore germination of were investigated. Cycloheximide (3.55 μm) inhibited spore germination when added during the first 40 min of incubation, but was ineffective when added after 40 min. Spores germinated when cycloheximide was removed from the suspension for 2 h during incubation, regardless of the length of pretreatment with cycloheximide. By pulse-labelling with [C]glycine, it was evident that the protein which was synthesized during the first 40 min of incubation was indispensable for the initiation of germination, but protein synthesis which occurred at a later stage of incubation was not necessary for spore germination directly.


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