1887

Abstract

During spore development of both protein synthesis and sporulation become resistant to the antibiotic fusidic acid. This resistance develops at the time when asymmetric prespore septa are formed. Simultaneously ribosomes lose their ability to bind fusidic acid, as demonstrated by their affinity chromatography with the immobilized drug. Mutants resistant to fusidic acid during growth are oligosporogenous; their sporulation development is blocked before septum formation. These results indicate that normal ribosomes are needed for prespore septation sporulation; only after septation can protein synthesis be maintained, throughout the development period, by fusidate resistant ribosomes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-101-2-299
1977-08-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/101/2/mic-101-2-299.html?itemId=/content/journal/micro/10.1099/00221287-101-2-299&mimeType=html&fmt=ahah

References

  1. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  2. Chopra I. 1976; Mechanisms of resistance to fusidic acid in Staphylococcus aureus. Journal of General Microbiology 96:229–238
    [Google Scholar]
  3. Cundliffe E. 1972; The mode of action of fusidic acid. Biochemical and Biophysical Research Communications 46:1794–1801
    [Google Scholar]
  4. Fortnagel P. 1973; Ribosomal alteration during sporulation of Bacillus subtilis. Colloques internationaux du Centre national de la recherchescientifique 227:113–114
    [Google Scholar]
  5. Fortnagel P. 1977; Affinity chromatographic analysis of ribosomal changes during sporulation of B. subtilis.. In Spore Research 1976. Barker A. N., Wolf J., Ellar D. J., Dring G. J., Gould G. W. Edited by London: Academic Press; in the Press
    [Google Scholar]
  6. Fortnagel P., Bergmann R. 1973; Alteration of the ribosomal fraction of Bacillus subtilis during sporulation. Biochimica et biophysica acta 299:136–141
    [Google Scholar]
  7. Fortnagel P., Bergmann R., Hafemann B., Lengelsen C. 1975; Structural and functional alteration of Bacillus subtilis ribosomes. In Spores VI pp. 301–306 Gerhardt P., Sadoff H. L., Costillow R. N. Edited by Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  8. Freese E., Fortnagel P. 1967; Analysis of sporulation mutants. I. Response of uracil incorporation to carbon sources and other mutant properties. Journal of Bacteriology 94:1957–1969
    [Google Scholar]
  9. Freese E. B., Cooney P., Freese E. 1975; Conditions controlling commitment of differentiation in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America 72:4037–4041
    [Google Scholar]
  10. Kornberg A., Spudich J. A., Nelson D. L., Deutscher M. P. 1968; Origin of proteins in sporulation. Annual Review of Biochemistry 37:51–78
    [Google Scholar]
  11. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  12. Porath J. 1974; General methods and coupling procedures. Methods in Enzymology 34:13–30
    [Google Scholar]
  13. Ryter A., Schaeffer P., Ionesco H. 1966; Classification cytologique, par leurstade de blocage, des mutants de sporulation de Bacillus subtilis Marburg. Annales de l’Institut Pasteur 110:305–315
    [Google Scholar]
  14. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques internationaux du Centre national de la recherche scientifique 124:553–563
    [Google Scholar]
  15. Sherwood M. B., Falco E. A., De Beer E. J. 1944; Factors influencing the speed and accuracy of a simple quantitative assay method of testing antibiotic substances. Journal of Bacteriology 47:424
    [Google Scholar]
  16. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
/content/journal/micro/10.1099/00221287-101-2-299
Loading
/content/journal/micro/10.1099/00221287-101-2-299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error