1887

Abstract

The rumen ciliate protozoon has been grown in the presence of bacteria and for over a year at population densities of 100 to 200 ml. The medium contained potassium phosphate, prepared fresh rumen fluid, cysteine, wholemeal flour (or rice starch), dried grass and a culture of the spineless form of has an obligate requirement for this protozoon and died within 48 h in its absence. During growth from a 2% inoculum, the mean generation time of was 6 h. engulfed 1·5 to 2·5 organisms h, and when was in excess it developed caudal spines for the first time in 17 years; these spined forms were engulfed much less readily than the spineless organisms.

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/content/journal/micro/10.1099/00221287-101-2-253
1977-08-01
2024-12-12
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References

  1. Coleman G. S. 1958; Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature; London: 1821104–1105
    [Google Scholar]
  2. Coleman G. S. 1960; The cultivation of sheep oligotrich protozoa in vitro. Journal of General Microbiology 22:555–563
    [Google Scholar]
  3. Coleman G. S. 1964; The metabolism of Escherichia coli and other bacteria by Entodinium caudatum. Journal of General Microbiology 37:209–223
    [Google Scholar]
  4. Coleman G. S. 1969a; The cultivation of the rumen ciliate Entodinium simplex. Journal of General Microbiology 57:81–90
    [Google Scholar]
  5. Coleman G. S. 1969b; The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudatum. Journal of General Microbiology 57:303–332
    [Google Scholar]
  6. Coleman G. S. 1971; The cultivation of rumen Entodiniomorphid protozoa. In Isolation of Anaerobes pp. 159–176 Shapton D. A., Board R. B. Edited by London and New York: Academic Press;
    [Google Scholar]
  7. Coleman G. S. 1972; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rùmen ciliate Entodinium simplex. Journal of General Microbiology 71:117–131
    [Google Scholar]
  8. Coleman G. S., Davies J. I., Cash M. A. 1972; The cultivation of the rumen ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro. Journal of General Microbiology 73:509–521
    [Google Scholar]
  9. Coleman G. S., Laurie J. I., Bailey J. E., Holdgate S. A. 1976; The cultivation of cellulolytic protozoa isolated from the rumen. Journal of General Microbiology 95:144–150
    [Google Scholar]
  10. Dogiel V. A. 1927; Monographie der Familie Ophryoscolecidae. Archiv für Protistenkunde 59:1–227
    [Google Scholar]
  11. Eadie J. M. 1970; Rumen ciliate ecology. Rowett Research Institute, Annual Report of Studies in Animal Nutrition and Allied Sciences 26:34–35
    [Google Scholar]
  12. Kofoid C. A., Maclennan R. F. 1930; Ciliates from Bos indicus Linn. I. The genus Entodinium Stein. University of California Publications in Zoology 33:471–544
    [Google Scholar]
  13. Kudo R. R. 1946 Protozoology. Illinois, U.S.A.: C. C. Thomas;
    [Google Scholar]
  14. Owen R. W., Coleman G. S. 1976; The cultivation of the rumen ciliate Entodinium longinucleatum. Journal of Applied Bacteriology 41:341–344
    [Google Scholar]
  15. Poljansky G., Strelkow A. 1938; Étude expérimentale sur la variabilité de quelques Ophryoscolécidés. Archives de zoologie Expérimentale et générale 80:1–123
    [Google Scholar]
  16. Stein F. 1858; Ueber mehere neue im Pansen der Wiederkauer lebende Infusionsthiere. Abhandlungen der Kaiserliche Böhmischen Gesellschaft der Wissenschaften 10:69–70
    [Google Scholar]
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