1887

Abstract

The rumen ciliate protozoon has been grown in the presence of bacteria and for over a year at population densities of 100 to 200 ml. The medium contained potassium phosphate, prepared fresh rumen fluid, cysteine, wholemeal flour (or rice starch), dried grass and a culture of the spineless form of has an obligate requirement for this protozoon and died within 48 h in its absence. During growth from a 2% inoculum, the mean generation time of was 6 h. engulfed 1·5 to 2·5 organisms h, and when was in excess it developed caudal spines for the first time in 17 years; these spined forms were engulfed much less readily than the spineless organisms.

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/content/journal/micro/10.1099/00221287-101-2-253
1977-08-01
2021-07-24
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