Summary: When Tween 80 (polyoxyethylene sorbitan mono-oleate) was given as a supplement in basal ammonium sulphate/glucose medium to the yeast , it induced the development, throughout growth, of populations consisting almost entirely of triangular cells. This induction was inhibited by an increase in growth temperature and by adding specific concentrations of short-chain alcohols to the medium. Only Tween 80 among the Tween detergents and Span 80 (sorbitan mono-oleate) among the Span detergents induced the formation of triangular cells, and Brij 96 (polyoxyethylene-10-oleyl ether) and sodium oleate were ineffective. These results indicate that the ester-linked sorbitan oleate is the chemical characteristic which is responsible for the effect of Tween on the morphology of , in a system more reproducible than any other previously investigated.


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