1887

Abstract

A cellulolytic bacterium, identified as belonging to the genus , produced two classes of cellulases when grown in the presence of cellulose. One class comprised enzymes which were tightly bound to cellulose, and the other, enzymes which were found free in the culture supernatant fluid. The cellulose-bound activity reached a maximum at the beginning of the stationary phase and then decreased, whereas the soluble activity increased to a plateau late in the stationary phase. The production of both classes of enzyme was repressed by adding cellobiose or glycerol to the cellulose. Deprivation of carbon sources did not induce cellulolytic activity. Cellobiose and sophorose showed only weak inducing activity in the absence of cellulose.

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/content/journal/micro/10.1099/00221287-101-2-191
1977-08-01
2021-05-12
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