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Mycobacterium smegmatis formed an alkaline phosphomonoesterase (EC 3·1·3·1) and an alkaline phosphodiesterase (EC 3·1·4·1). The former was regulated by inorganic phosphate and was repressed in medium containing a high concentration of phosphate. The synthesis and the function of the alkaline phosphodiesterase were not controlled by inorganic phosphate.
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