1887

Abstract

The presence in recipient strains of 12 of the plasmid R46 greatly reduced the yield of recombinants from crosses with several Hfr strains and virtually abolished the formation of recombinants by P1 transduction without, however, significantly affecting the transfer of the F prime from a strain carrying F. The R46 plasmid had paradoxical effects on mutability: it appeared to enhance the yield of mutants following irradiation with ultraviolet light but it reduced the number of mutants detectable in unirradiated cultures. The effects of this plasmid on ultraviolet light survival of the wild type and several mutants defective for recombination and repair have been measured and the results, in the main, confirm similar observations by Tweats (1976) . Not only is the survival of the strain harbouring R46 greater than that of the parent strain in all the cases studied, but the survival of ultraviolet irradiated bacteriophage λ is also greater.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-101-1-93
1977-07-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/101/1/mic-101-1-93.html?itemId=/content/journal/micro/10.1099/00221287-101-1-93&mimeType=html&fmt=ahah

References

  1. Braun A., Grossman L. 1974; An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from uvrA and uvrB mutants. Proceedings of the National Academy of Sciences of the United States of America 71:1838–1842
    [Google Scholar]
  2. Clark A. J. 1973; Recombination deficient mutants of E. coli and other bacteria. Annual Review of Genetics 7:67–86
    [Google Scholar]
  3. Drabble W. T., Stocker B. A. D. 1968; R (transmissible drug-resistance) factors in Salmonella typhimurium: pattern of transduction by phage P22 and ultraviolet protection effect. Journal of General Microbiology 53:109–123
    [Google Scholar]
  4. Freifelder D. 1969; Single strand breaks in bacterial DNA associated with thymine starvation. Journal of Molecular Biology 45:1–7
    [Google Scholar]
  5. Grossman L., Braun A., Feldberg R., Mahler I. 1975; Enzymatic repair of DNA. Annual Review of Biochemistry 44:19–43
    [Google Scholar]
  6. Hedges R. W. 1972; Phenotypic characterisation of fi R factors determining the restriction and modification hspll specificity. Molecular and General Genetics 115:225–233
    [Google Scholar]
  7. Howarth S. 1965; Resistance to the bactericidal effect of ultraviolet radiation conferred on enterobacteria by the colicine factor colI. Journal of General Microbiology 40:43–55
    [Google Scholar]
  8. Krishnapillai V. 1975; Resistance to ultraviolet light and enhanced mutagenesis conferred by Pseudomonas aeruginosa plasmids. Mutation Research 29:363–372
    [Google Scholar]
  9. Lloyd R. G. 1971; Recombination deficient mutants of Escherichia coli K12. Ph.D. thesis University of Sussex;
    [Google Scholar]
  10. Macphee D. 1974; DNA polymerase activity determined by the ultraviolet protecting activity of plasmid R-Utrecht. Nature; London: 251432–434
    [Google Scholar]
  11. Marsh E. B., Smith D. H. 1969; R-factors improving survival of Escherichia coli K12 aftor ultraviolet irradiation. Journal of Bacteriology 100:128–139
    [Google Scholar]
  12. Pinney R. J., Bremer K., Smith J. T. 1974; R factor elimination by inhibition of thymidylatesynthetase (fluorodeoxyavidine and showdomycin) and the occurrence of SS breaks in plasmid DNA. Molecular and General Genetics 133:163–174
    [Google Scholar]
  13. Siccardi A. G. 1969; Effect of R factors and other plasmids on ultraviolet susceptibility and host-cell reactivation property of Escherichia coli. Journal of Bacteriology 100:337–346
    [Google Scholar]
  14. Signer E. R., Weil J. 1968; Recombination in bacteriophage λ. I. Mutants deficient in general recombination. Journal of Molecular Biology 34:261–271
    [Google Scholar]
  15. Stacey K. A., Evenchik Z., Hayes W. 1969; The effects of ultraviolet irradiation on the fertility of F+ and Hfr strains of Escherichia coli K12 defective for the repair of damaged DNA. Journal of General Microbiology 59:185–191
    [Google Scholar]
  16. Stacey K. A., Oliver P. 1977; A novel pleiotropic mutation in Escherichia coli K12 which affects transduction, transformation and rates of mutation. Journal of General Microbiology 98:569–578
    [Google Scholar]
  17. Trgovcevic Z., Rupp W. D. 1974; Interaction of bacterial and lambda phage recombination systems in the X-ray sensitivity of Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 71:503–506
    [Google Scholar]
  18. Tweats D. J., Smith J. T. 1974; R-factor mediated nuclease activity involved in thymineless elimination. Journal of Bacteriology 118:790–795
    [Google Scholar]
  19. Tweats D. J., Thompson M. J., Pinney R. J., Smith J. T. 1976; R factor-mediated resistance to ultraviolet light in strains of Escherichia coli deficient in known repair functions. Journal of General Microbiology 93:103–110
    [Google Scholar]
  20. Zissler J., Signer E., Schafer F. 1971; The role of recombination in the growth of bacteriophage lamda. I. The gamma gene. In The Bacteriophage Lambda pp. 455–468 Hershey A. D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-101-1-93
Loading
/content/journal/micro/10.1099/00221287-101-1-93
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error