1887

Abstract

In blue light assimilates less CO and excretes less citric acid into the medium than when grown in continuous darkness. This is due to a decreased supply of pyruvate which is carboxylated to form citric acid via oxaloacetate. Pyruvate carboxylase rather than phosphoenolpyruvate carboxylase or phosphoenolpyruvate carboxykinase is mainly responsible for CO assimilation in this fungus. Of the glycolytic intermediates assayed, steady-state concentrations of pyruvate were low and concentrations of glucose 6-phosphate, glucose 1-phosphate and fructose 6-phosphate were high in mycelium grown in the light compared with those in mycelium grown in the dark. Results of experiments with specifically-labelled glucose suggest that light stimulates the pentose phosphate pathway and inhibits the supply of pyruvate for citric acid synthesis via the Embden-MeyerhofParnas pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-101-1-157
1977-07-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/101/1/mic-101-1-157.html?itemId=/content/journal/micro/10.1099/00221287-101-1-157&mimeType=html&fmt=ahah

References

  1. Bergmeyer H. U. 1963 Methods of Enzymatic Analysis New York and London: Academic Press;
    [Google Scholar]
  2. Budd K. 1971; Bicarbonate fixation by cell-free extracts and by mycelium of Neocosmospora vasinfecta . Journal of General Microbiology 67:99–106
    [Google Scholar]
  3. Cannata J. J. B., Stoppani A. O. M. 1963; Phosphopyruvate carboxylase from bakers’ yeast. Journal of Biological Chemistry 238:1196–1207
    [Google Scholar]
  4. Cleland K. W., Slater E. C. 1953; Respiratory granules of heart muscle. Biochemical Journal 53:547–556
    [Google Scholar]
  5. Estabrook R. W., Maitra P. K. 1962; A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides. Analytical Biochemistry 3:369–382
    [Google Scholar]
  6. Feir H. A., Suzuki I. 1969; Pyruvate carboxylase of Aspergillus niger: kinetic study of a biotin-containing carboxylase. Canadian Journal of Biochemistry 47:697–710
    [Google Scholar]
  7. Graafmans W. D. J. 1973a; The influence of carbon dioxide on morphogenesis in Penicillium isariiforme . Archiv für Mikrobiologie 91:67–71
    [Google Scholar]
  8. Graafmans W. D. J. 1973b; The influence of carbon dioxide on sporulation and citric acid synthesis in dark-grown Penicillium isariiforme . Acta botanica neerlandica 22:543–545
    [Google Scholar]
  9. Graafmans W. D. J. 1974; Metabolism in Penicillium isariiforme on exposure to light, with special reference to citric acid synthesis. Journal of General Microbiology 82:247–252
    [Google Scholar]
  10. Graafmans W. D. J. 1976 Enige biochemische aspecten van de morfogenese bij Penicillium isarii-forme. Ph.D. thesis University of Amsterdam.:
    [Google Scholar]
  11. Maitra P. K., Estabrook R. W. 1964; A fluorimetric method for the enzymatic determination of glycolytic intermediates. Analytical Biochemistry 7:472–484
    [Google Scholar]
  12. Ng W. S., Smith J. E., Anderson J. G. 1972; Changes in carbon catabolic pathways during synchronous development of conidiophores of Aspergillus niger . Journal of General Microbiology 71:495–504
    [Google Scholar]
  13. Ruiz-Amil M., De Torrontegui G., Palacián E., Catalina L., Losada M. 1965; Properties and function of yeast pyruvate carboxylase. Journal of Biological Chemistry 240:3485–3492
    [Google Scholar]
  14. Turian G., Bianchi D. E. 1972; Conidiation in Neurospora . Botanical Review 38:119–154
    [Google Scholar]
  15. Utter M. F., Keech D. B. 1960; Formation of oxaloacetate from pyruvate and CO2 . Journal of Biological Chemistry 235:PC 17
    [Google Scholar]
  16. Wang C. H. 1972; Radiorespirometric methods. In Methods in Microbiology pp. 185–230 Norris J. R., Ribbons D. W. Edited by New York and London: Academic Press;
    [Google Scholar]
  17. Wong A. L., Willets H. J. 1974; Polyacrylamide-gel electrophoresis of enzymes during morphogenesis of sclerotia of Sclerotinia sclerotiorum . Journal of General Microbiology 81:101–109
    [Google Scholar]
  18. Wright B. E. 1966; Multiple causes and controls in differentiation. Science 153:830–837
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-101-1-157
Loading
/content/journal/micro/10.1099/00221287-101-1-157
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error