1887

Microbiology Society logo

Volume 100, Issue 2

A Factor Involved in Chloramphenicol Resistance in 3(2): Its Transfer in the Absence of the Fertility Factor Free

Abstract

Summary

An element controlling chloramphenicol resistance () was detected in 3(2). Strains sensitive to 1 g chloramphenicol ml were obtained among dark scarlet variants. Transfer of the resistance factor was attempted in matings between chloramphenicol-resistant (Chl) and chloramphenicol-sensitive (Chl) strains, both of which lacked the SCP fertility factor. Transfer of was obtained at a much higher rate than that expected for chromosomal markers in SCP1 × SCP1 matings. However, in these particular crosses the latter was also several times higher than usual. All recombinants for chromosomal markers were Chl. Attempts to locate the element failed to distinguish between a chromosomal and an extrachromosomal site. The observed increase in the recombination frequency for chromosomal markers suggests that the element may promote recombination.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1977
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-2-347
1977-06-01
2023-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/2/mic-100-2-347.html?itemId=/content/journal/micro/10.1099/00221287-100-2-347&mimeType=html&fmt=ahah

References

  1. Akagawa H., Okanishi M., Umezawa H. 1975; A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping. Journal of General Microbiology 90:336–346
     [Google Scholar]
  2. Argoudelis A. D., Coats J. H. 1971; Microbial transformation of antibiotics.VI.Acylation of chloramphenicol by Streptomyces coelicolor. . Journal of Antibiotics 24:206–208
     [Google Scholar]
  3. Gregory K. F., Shyu W. J. 1961; Apparent cytoplasmic inheritance of tyrosinase competence in Streptomyces scabies. . Nature; London: 191465–467
     [Google Scholar]
  4. Hopwood D. A., Sermonti G. 1962; The Genetics of Streptomyces coelicolor. . Advances in Genetics 11:273–342
     [Google Scholar]
  5. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
     [Google Scholar]
  6. Kirby R., Wright L. F., Hopwood D. A. 1975; Plasmid determined antibiotic synthesis and resistance in Streptomyces coelicolor. . Nature; London: 254265–267
     [Google Scholar]
  7. Puglia A. M., Spada-Sermonti I., Basile S., Misuraca F., Sermonti G. 1973; Infectious transfer of a fertility factor in Streptomyces coelicolor. . Genetical Research, Cambridge 21:107–113
     [Google Scholar]
  8. Sermonti G. 1975a; Plasmids in Streptomycetes. . Microbial Genetics Bulletin 39:10–11
     [Google Scholar]
  9. Sermonti G. 1975b; Resistance Factor in Streptomyces coelicolor A3(2). Microbial Genetics Bulletin 39:5–6
     [Google Scholar]
  10. Shaw W. V., Hopwood D. A. 1976; Chloramphenicol acetylation in Streptomyces. . Journal of General Microbiology 94:159–166
     [Google Scholar]
  11. Vining L. C. 1975 Methods in Enzymology 43:734–737
     [Google Scholar]
  12. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor a(3)2: plasmid involvement in the interconversion of UF and IF strains. Journal of General Microbiology 69:353–364
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-2-347
Loading
A Factor Involved in Chloramphenicol Resistance in Streptomyces coelicolora3(2): Its Transfer in the Absence of the Fertility Factor
Microbiology 100, 347 (1977); https://doi.org/10.1099/00221287-100-2-347
/content/journal/micro/10.1099/00221287-100-2-347
/content/journal/micro/10.1099/00221287-100-2-347
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error