RT Journal Article SR Electronic(1) A1 Edwards, Clive A1 Lloyd, DavidYR 1977 T1 Subcellular Fractionation by Differential and Zonal Centrifugation of the Trypanosomatid Crithidia fasciculata JF Microbiology, VO 100 IS 2 SP 339 OP 346 DO https://doi.org/10.1099/00221287-100-2-339 PB Microbiology Society, SN 1465-2080, AB Summary Homogenates of Crithidia fasciculata were fractionated by differential centrifugation. Mitochondria were sedimented quantitatively at 104 g-min and accounted for approximately 10% of the total recovered protein. Catalase was found exclusively in the supernatant fraction whilst NADH: cytochrome c oxidoreductase and p-nitrophenylphosphatase were found in all the fractions. Zonal centrifugation confirmed that catalase was non-sedimentable. Clean separation of mitochondria was obtained in both high-speed and rate zonal experiments, but no NADH: cytochrome c oxidoreductase activity could be detected in these organelles. Separation of large lysosomal vacuoles which contained p-nitrophenylphosphatase activity was obtained and these were clearly resolved from mitochondria by both highspeed and rate zonal centrifugation., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-100-2-339