1887

Abstract

Summary

Homogenates of were fractionated by differential centrifugation. Mitochondria were sedimented quantitatively at 10 -min and accounted for approximately 10% of the total recovered protein. Catalase was found exclusively in the supernatant fraction whilst NADH: cytochrome oxidoreductase and -nitrophenylphosphatase were found in all the fractions. Zonal centrifugation confirmed that catalase was non-sedimentable. Clean separation of mitochondria was obtained in both high-speed and rate zonal experiments, but no NADH: cytochrome oxidoreductase activity could be detected in these organelles. Separation of large lysosomal vacuoles which contained -nitrophenylphosphatase activity was obtained and these were clearly resolved from mitochondria by both highspeed and rate zonal centrifugation.

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/content/journal/micro/10.1099/00221287-100-2-339
1977-06-01
2021-05-06
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