1887

Abstract

Summary

The electron transfer system catalysing reduction of fumarate by NADH under anaerobic conditions in membrane preparations of strain 4 was inhibited by 2-n-heptyl-4-hydroxyquinoline -oxide. Reduction of -type cytochrome by NADH under anaerobic conditions was much slower than reduction of fumarate by NADH, and was more sensitive to 2-n-heptyl-4-hydroxyquinoline -oxide inhibition. Thus -type cytochrome is probably not an intermediate in the reduction of fumarate by NADH under the assay conditions used. Extraction of quinone from the membrane and subsequent reincorporation showed that menaquinone (vitamin K) is an obligatory intermediate in the reduction of both fumarate and -type cytochrome by NADH, which is consistent with the 2-n-heptyl-4-hydroxyquinoline -oxide inhibition results. A tentative scheme for electron transport in is presented.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-2-309
1977-06-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/2/mic-100-2-309.html?itemId=/content/journal/micro/10.1099/00221287-100-2-309&mimeType=html&fmt=ahah

References

  1. Broberg P. L., Smith L. 1967; The cytochrome system of Bacillus megaterium KM. The presence and some properties of two CO-binding cytochromes. Biochimica et biophysica acta 131:479–489
    [Google Scholar]
  2. Bryant M. P., Small N., Bouma C., Chu H. 1958; Bacteroides ruminicola n.sp. and Succinimonas amylolytica the new genus and species. Species of succinic acid-producing anaerobic bacteria of the bovine rumen. Journal of Bacteriology 76:15–23
    [Google Scholar]
  3. Chance B. 1952; Spectra and reaction kinetics of respiratory pigments of homogenised and intact cells. Nature; London; 169215–221
    [Google Scholar]
  4. Cox G. B., Newton N. A., Gibson F., Snoswell A. M., Hamilton J. A. 1970; The function of ubiquinone in Escherichia coli. . Biochemical Journal 117:551–562
    [Google Scholar]
  5. Crane F. L., Sun F. F. 1972; Lipid and lipid-soluble compounds in electron transfer systems. In Electron and Coupled Energy Transfer in Biological Systems 1B pp. 477–587 King T. E., Klingenberg M. Edited by New York: Marcel Dekker;
    [Google Scholar]
  6. Deeb S. S., Hager L. P. 1964; Crystalline cytochrome b 1 from Escherichia coli. . Journal of Biological Chemistry 239:1024–1031
    [Google Scholar]
  7. Ernster L., Lee I. Y., Norling B., Persson B. 1969; Studies with ubiquinone-depleted submitochondrial particles. Essentiality of ubiquinone for the interaction of succinate dehydrogenase, NADH dehydrogenase and cytochrome b. . European Journal of Biochemistry 9:299–310
    [Google Scholar]
  8. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  9. Hobson P. N., Summers R. 1967; The continuous culture of anaerobic bacteria. Journal of General Microbiology 47:53–65
    [Google Scholar]
  10. Howlett M. R., Mountfort D. O., Turner K. W., Roberton A. M. 1976; Metabolism and growth yields in Bacteroides ruminicola strain B14. Applied and Environmental Microbiology 32:274–283
    [Google Scholar]
  11. Kroger A., Dadak V. 1969; On the role of quinones in bacterial electron transport. The respiratory system of Bacillus megaterium. . European Journal of Biochemistry 11:328–340
    [Google Scholar]
  12. Kroger A., Dadak V., Klingenberg M., Diemer F. 1971; On the role of quinones in bacterial electron transport. Differential roles of ubiquinone and menaquinone in Proteus rettgeri. . European Journal of Biochemistry 21:322–333
    [Google Scholar]
  13. Macy J., Probst I., Gottschalk B. 1975; Evidence for cytochrome involvement in fumarate reduction and ATP synthesis by Bacteroides fragilis grown in the presence of haem. Journal of Bacteriology 123:436–442
    [Google Scholar]
  14. Morton R. A. 1965; Spectroscopy of quinones and related substances. I. Ultraviolet absorption spectra. In Biochemistry of Quinones pp. 23–65 Morton R. A. Edited by New York and London: Academic Press;
    [Google Scholar]
  15. Reddy C. A., Bryant M. P. 1967; DNA base composition and cytochromes in certain species of the genus Bacteroides. . Bacteriological Proceedings, p.40
    [Google Scholar]
  16. Schwartz A. C., Sporkenbach J. 1975; The electron transport system of the anaerobic Propioni-bacterium shermanii. Cytochrome and inhibitor studies. Archives of Microbiology 102:261–273
    [Google Scholar]
  17. Shipp W. S. 1972; Absorption bands of multiple b and c cytochromes in bacteria detected by numerical analysis of absorption spectra. Archives of Biochemistry and Biophysics 150:482–488
    [Google Scholar]
  18. De Vries W., Van Wyck-Kapteyn W. M. C., Stouthamer A. H. 1973; Generation of ATP during cytochrome-linked anaerobic electron transport in propionic acid bacteria. Journal of General Microbiology 76:31–41
    [Google Scholar]
  19. White D. C., Bryant M. P., Caldwell D. R. 1962; Cytochrome-linked fermentation in Bacteroides ruminicola. . Journal of Bacteriology 84:822–828
    [Google Scholar]
/content/journal/micro/10.1099/00221287-100-2-309
Loading
/content/journal/micro/10.1099/00221287-100-2-309
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error