1887

Abstract

Summary

Attempts were made to obtain recombinants between the morganocinogenic factor Mor174 and R plasmid R772 by transductional techniques. Transduction frequencies of the kanamycin resistance marker of R772 to 5006 or Providence 29 carrying Mor174 by phages 5006M and PL25, respectively, were 1000-fold higher than to the corresponding Mor174 strains. The frequency of phage P1-mediated transduction of the same marker to J62 was high and was not affected by the presence of Mor174 in the recipient. Transduction of the kanamycin resistance marker to 5006 yielded heterogenote-like transductants. Transduction of the same marker to 5006 with Mor174 as resident resulted in transductants which not only yielded low frequency kanamycin resistance transducing particles on induction but could transfer Mor174 and the transduced marker independently by conjugation. It was suggested that Mor174 exerted some function in establishing a transductionally shortened R factor. Mor174 also possibly played a role in the separation of the phage component of the transducing particle from the R factor portion. The severed phage genes could then integrate in tandem to a cryptic prophage to render transductants inducible. A phage 5006M lysate of 5006(Mor174R772) produced a cotransductant. This transductant could not, possibly due to more extensive transductional shortening, transfer markers by conjugation. Induction yielded some particles which could cotransduce the markers to 5006(P-). With assistance of the latter conjugative plasmid, markers of Mor174 and R772 were transferred as a unit by conjugation to strain J62. Phage PI reared on the latter transconjugant cotransduced the genes for Mor174 activity and kanamycin resistance. Morganocin production by the recombinant equalled that of Mor174.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-2-299
1977-06-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/2/mic-100-2-299.html?itemId=/content/journal/micro/10.1099/00221287-100-2-299&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1956; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
    [Google Scholar]
  2. Arber W. 1960; Transduction of chromosomal genes and episomes in Escherichia coli. . Virology 11:273–288
    [Google Scholar]
  3. Clowes R. C. 1972; Molecular structure of bacterial plasmids. Bacteriological Reviews 36:361–405
    [Google Scholar]
  4. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics p. 227 Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  5. Coetzee J. N. 1974a; Properties of Proteus and Providence strains harbouring recombinant plasmids between P-lac and RIdrdig or R447b. Journal of General Microbiology 80:119–130
    [Google Scholar]
  6. Coetzee J. N. 1974b; High frequency transduction of kanamycin resistance in Proteus mirabilis. . Journal of General Microbiology 84:285–296
    [Google Scholar]
  7. Coetzee J. N. 1975; Transduction of a Proteus vulgaris strain by a Proteus mirabilis bacteriophage. Journal of General Microbiology 89:299–309
    [Google Scholar]
  8. Coetzee J. N., Smit J. A. 1970; Properties of Proteus mirabilis phage 13vir. . Journal of General Virology 9:247–249
    [Google Scholar]
  9. Coetzee J. N., Smit J. A., Prozesky O. W. 1966; Properties of Providence and Proteus morganii% transducing phages. Journal of General Microbiology 44:167–176
    [Google Scholar]
  10. Coetzee J. N., Datta N., Hedges R. W. 1972; R Factors from Proteus rettgeri. . Journal of General Microbiology 72:543–552
    [Google Scholar]
  11. Coetzee J. N., Datta N., Hedges R. W., Appelbaum P. C. 1973; Transduction of R factors in Proteus mirabilis P. rettgeri. . Journal of General Microbiology 76:355–368
    [Google Scholar]
  12. Cooper P. 1971; Interaction of a colicinogenic factor with a resistance factor and with the fertility factor F in Escherichia coli K-12. Genetical Research 17:151–159
    [Google Scholar]
  13. Fredericq P., Delhalle E. 1972; Recombinaison entre facteurs R et facteurs colicinogenes chez Escherichia coli KI2. Annales de l’Institut Pasteur 122:909–921
    [Google Scholar]
  14. Hedges R. W. 1975; R factors from Proteus mirabilis P. vulgaris. . Journal of General Microbiology 87:301–311
    [Google Scholar]
  15. Hedges R. W., Datta N. 1971; fi R factors giving chloramphenicol resistance. Nature; London: 234220–221
    [Google Scholar]
  16. Hedges R. W., Datta N., Coetzee J. N., Dennison S. 1973; R factors from Proteus morganii. . Journal of General Microbiology 77:249–259
    [Google Scholar]
  17. Hedges R. W., Jacob A., Datta N., Coetzee J. N. 1975; Properties of plasmids produced by recombination between R factors of groups J and FII. Molecular and General Genetics 140:289–302
    [Google Scholar]
  18. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. 1974; Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  19. Ikeda H., Tomizawa J.-I. 1965; Transducing fragments in generalized transduction by phage Pi. I. Molecular origin of the fragments. Journal of Molecular Biology 14:85–109
    [Google Scholar]
  20. Ikeda H., Tomizawa J.-I. 1968; Prophage PI, an extrachromosomal replication unit. Cold Spring Harbor Symposia on Quantitative Biology 33:791–798
    [Google Scholar]
  21. Kameda M., Harada K., Suzuki M., Mitsuhashi S. 1969; Formation of transferable drug resistance factor by recombination between resistance determinants and transfer factors. Japanese Journal of Microbiology 13:255–262
    [Google Scholar]
  22. Krizsanovich K. 1973; Cryptic lysogeny in Proteus mirabilis. . Journal of General Virology 19:311–320
    [Google Scholar]
  23. Lovett M. A., Helinski D. R. 1976; Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F ’km plasmid. Journal of Bacteriology 127:982–987
    [Google Scholar]
  24. Luria S. E., Adams J. N., Ting R. C. 1960; Transduction of lactose-utilizing ability among strains of E. coli S. dysenteriae and the properties of the transducing phage particles. Virology 12:348–390
    [Google Scholar]
  25. Meynell E., Cooke M. 1969; Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC. Genetical Research 14:309–313
    [Google Scholar]
  26. Meynell E., Datta N. 1966; The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli KI2. Genetical Research 7:134–140
    [Google Scholar]
  27. Meynell E., Datta N. 1967; Mutant drug resistance factors of high transmissibility. Nature; London : 214885–887
    [Google Scholar]
  28. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:55–83
    [Google Scholar]
  29. Meynell G. G. 1969; Exclusion, superinfection immunity and abortive recombinants in I+×I+ bacterial crosses. Genetical Research 13:113–115
    [Google Scholar]
  30. Ozeki H., Stocker B. A. D. 1958; Phage-mediated transduction of colicinogeny in Salmonella typhi-murium. . Heredity 12:525–526
    [Google Scholar]
  31. Schmieger H. 1970; The molecular structure of the transducing particles of Salmonella phage P22. II. Density gradient analysis of DNA. Molecular and General Genetics 109:323–337
    [Google Scholar]
  32. Shipley P. L., Olsen R. H. 1975; Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RPi . Journal of Bacteriology 123:20–27
    [Google Scholar]
  33. Smith H. R., Humphreys G. O., Willshaw G. A., Anderson E. S. 1976; Characterisation of plasmids coding for the restriction endonuclease Eco% R1. Molecular and General Genetics 143:319–325
    [Google Scholar]
  34. Watanabe T., Ogata Y. 1970; Abortive transduction of resistance factor by bacteriophage P22 in Salmonella typhimurium. . Journal of Bacteriology 102:596–597
    [Google Scholar]
  35. Watanabe T., Sakaizumi S., Furuse C. 1968; superinfection with R factors by transduction in Escherichia coli Salmonella typhimurium. . Journal of Bacteriology 96:1796–1802
    [Google Scholar]
  36. Williams J. A. 1977; Mobilization of morganocin 174 plasmid and kinetics of morganocin production in Proteus and Escherichia coli hosts. Antimicrobial Agents and Chemotherapy in the Press
    [Google Scholar]
  37. Yamamoto K. R., Alberts B. M. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-2-299
Loading
/content/journal/micro/10.1099/00221287-100-2-299
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error