1887

Abstract

SUMMARY: Mutants of 10015 were isolated which produced catechol 2,3-oxygenase and the subsequent enzymes of the -cleavage pathway constitutively, and were defective in phenol hydroxylase activity. All revertants of one mutant (C), and most revertants of a second mutant (C), regained wild-type characteristics with respect to inducibility of phenol hydroxylase and the other -cleavage enzymes. Their behaviour was consistent with them being regulatory mutants.

Other mutants deficient in phenol hydroxylase activity were not constitutive for catechol 2,3-oxygenase and other enzymes of the pathway. Their properties were consistent with mutations in a structural gene.

A model in which phenol hydroxylase is under positive control and catechol 2,3-oxygenase and subsequent enzymes are under negative control is proposed for the regulation of enzymes of the -cleavage pathway in .

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/content/journal/micro/10.1099/00221287-100-1-81
1977-05-01
2021-10-27
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References

  1. Bayly R. C., Wigmore G. J. 1973; Metabolism of phenol and cresols by mutants ofPseudomonas putida. Journal of Bacteriology 113:1112–1120
    [Google Scholar]
  2. Bayly R. C., Wigmore G. J., Mckenzie D. I. 1977; Regulation of the enzymes of themeta-cleavage pathway ofPseudomonas putida: the regulon is composed of two operons. Journal of General Microbiology 100:71–79
    [Google Scholar]
  3. Calhoun D. H., Hatfield G. W. 1975; Autoregulation of gene expression. Annual Review of Microbiology 29:275–299
    [Google Scholar]
  4. Cánovas J. L., Ornston L, Stanier R. Y. 1967; Evolutionary significance of metabolic control systems. Science 156:1695–1699
    [Google Scholar]
  5. Englesberg E., Wilcox G. 1974; Regulation. Positive control. Annual Review of Genetics 8:219–242
    [Google Scholar]
  6. Feist C. F., Hegeman G. D. 1969; Phenol and benzoate metabolism byPseudomonasputida: regulation of tangential pathways. Journal of Bacteriology 100:869–877
    [Google Scholar]
  7. Goldberger R. F. 1974; Autogenous regulation of gene expression. Science 183:810–816
    [Google Scholar]
  8. Sala-Trepat J. M., Evans W. C. 1971; Themeta- cleavage of catechol byAzotobacter species: 4-oxalo-crotonate pathway. European Journal of Biochemistry 20:400–413
    [Google Scholar]
  9. Sala-Trepat J. M., Murray K., Williams P. A. 1972; The metabolic divergence in themeta-cleavage of catechols byPseudomonas putida ncib10015. Physiological significance and evolutionary implications. European Journal of Biochemistry 28:347–356
    [Google Scholar]
  10. Wigmore G. J., Bayly R. C. 1974; A mutant of Pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. Biochemical and Biophysical Research Communications 60:48–55
    [Google Scholar]
  11. Wigmore G. J., Bayly R. C. 1977; A partial order for genes determining enzymes of the meta-cleavage pathway in Pseudomonas putida. Journal of General Microbiology 100:65–69
    [Google Scholar]
  12. Wigmore G. J., Bayly R. C., Di Berardino D. 1974; Pseudomonas putida mutants defective in the metabolism of the products of meta-fission of catechol and its methyl analogues. Journal of Bacteriology 120:31–37
    [Google Scholar]
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