1887

Abstract

SUMMARY: In 10015 the gene determining phenol hydroxylase is in a separate operon from the genes determining catechol 2,3-oxygenase and subsequent enzymes of the -cleavage pathway.

In the wild-type strain, non-coordinate induction of phenol hydroxylase and catechol 2,3-oxygenase occurred when catechol, phenol or isomers of cresol were used as inducers. Phenol hydroxylase was repressed to a greater extent than catechol 2,3-oxygenase when induced by phenol in the presence of either benzoate or acetate. In a mutant deficient in catechol 2,3-oxygenase, 3- or 4-methylcatechol repressed formation of phenol hydroxylase but not of enzymes functioning later in the pathway.

Four mutants had an altered regulatory element that resulted in different effects on the expression of phenol hydroxylase and catechol 2,3-oxygenase genes when isomers of cresol were used as inducers.

Repression of enzyme induction by acetate, benzoate or 3- or 4-methylcatechol showed that catechol 2,3-oxygenase and subsequent -cleavage pathway genes form part of the same operon. Benzoate or 3- or 4-methylcatechol repressed phenol hydroxylase but none of the other enzymes in the pathway, while acetate coordinately repressed catechol 2,3-oxygenase and subsequent enzymes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-1-71
1977-05-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/1/mic-100-1-71.html?itemId=/content/journal/micro/10.1099/00221287-100-1-71&mimeType=html&fmt=ahah

References

  1. Bayly R. C. 1969 The degradation of cresols by species of Pseudomonas Ph.D. thesis Monash University, Australia:
    [Google Scholar]
  2. Bayly R. C., Dagley S. 1969; Oxoenoic acids as metabolites in the bacterial degradation of catechols. Biochemical Journal 111:303–307
    [Google Scholar]
  3. Bayly R. C., Wigmore G. J. 1973; Metabolism of phenol and cresols by mutants of Pseudomonas putida. Journal of Bacteriology 113:1112–1120
    [Google Scholar]
  4. Bayly R. C., Dagley S., Gibson D. T. 1966; The metabolism of cresols by species of Pseudomonas. Biochemical Journal 101:293–301
    [Google Scholar]
  5. Collinsworth W. L., Chapman P. J., Dagley S. 1973; Stereospecific enzymes in the degradation of aromatic compounds by Pseudomonas putida. Journal of Bacteriology 113:922–931
    [Google Scholar]
  6. Dagley S. 1972; Microbial degradation of stable chemical structures: general features of metabolic pathways. In Degradation of Synthetic Organic Molecules in the Biosphere pp. 1–16 Washington, D.C: National Academy of Sciences;
    [Google Scholar]
  7. Feist C. F., Fiegeman G. D. 1969; Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. Journal of Bacteriology 100:869–877
    [Google Scholar]
  8. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes of the wild-type. Journal of Bacteriology 91:1140–1154
    [Google Scholar]
  9. Kemp M. B., Hegeman G. D. 1968; Genetic control of the β-ketoadipate pathway in Pseudomonas aeruginosa. Journal of Bacteriology 96:1488–1499
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1950; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. Maas W. K., Clark A. J. 1964; Studies on the mechanism of repression of arginine biosynthesis, II. Dominance of repressibility in diploids. Journal of Molecular Biology 8:365–370
    [Google Scholar]
  12. Murray K., Williams P. A. 1974; Role of catechol and the methylcatechols as inducers of aromatic metabolism in Pseudomonas putida. Journal of Bacteriology 117:1153–1157
    [Google Scholar]
  13. Patel T. R., Gibson D. T. 1974; Purification and properties of (+)-cis-naphthalene dihydrodiol dehydrogenase of Pseudomonas putida. Journal of Bacteriology 119:879–888
    [Google Scholar]
  14. Reiner A. M. 1971; Metabolism of benzoic acid by bacteria: 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid is an intermediate in the formation of catechol. Journal of Bacteriology 108:89–94
    [Google Scholar]
  15. Ribbons D. W. 1970; Specificity of monohydric phenol oxidations by meta-cleavage pathways in Pseudomonas aeruginosa T1. Archiv für Mikrobiologie 74:103–115
    [Google Scholar]
  16. Sala-Trepat J. M., Murray K., Williams P. A. 1972; The metabolic divergence in the meta-cleavage of catechols by Pseudomonas putida ncib10015.Physiological significance and evolutionary implications. European Journal of Biochemistry 28:347–356
    [Google Scholar]
  17. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  18. Wheelis M. L. 1975; The genetics of dissimilatory pathways in Pseudomonas. Annual Review of Microbiology 29:505–524
    [Google Scholar]
  19. Wheelis M. L., Stanier R. Y. 1970; The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics 66:245–266
    [Google Scholar]
  20. Wigmore G. J. 1975 Metabolism of phenol and cresols by mutant strains of Pseudomonas putida Ph.D. thesis Monash University, Australia:
    [Google Scholar]
  21. Wigmore G. J., Bayly R. C. 1974; A mutant of Pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. Biochemical and Biophysical Research Communications 60:48–55
    [Google Scholar]
  22. Wigmore G. J., Bayly R. C. 1977; A partial order for genes determining enzymes of the meta-cleavage pathway in Pseudomonas putida. Journal of General Microbiology 100:65–69
    [Google Scholar]
  23. Wigmore G. J., Bayly R. C., Di Berardino D. 1974; Pseudomonas putida mutants defective in the metabolism of the products of meta-fission of catechol and its methyl analogues. Journal of Bacteriology 120:31–37
    [Google Scholar]
  24. Wigmore G. J., Diberardino D., Bayly R. C. 1977; Regulation of the enzymes of the meta-cleavage pathway of Pseudomonas putida: a regulatory model. Journal of General Microbiology 100:81–87
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-1-71
Loading
/content/journal/micro/10.1099/00221287-100-1-71
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error