Transmission of PsV-F and PsV-S Mycoviruses during Conidiogenesis of Free

Abstract

SUMMARY: Levels of both fast and slow virus (PsV-F and PsV-S) were evaluated in single conidial isolates of 5267. Approximately 7 % (3/43) of a random population of conidia from a culture of contained no PsV-F, while other conidia produced cultures with various levels of PsV-F. The greatest variation in PsV-F levels was observed in single conidial isolates from cultures that contained low to intermediate levels of virus, i.e. 11 to 25 units per g dry weight of mycelium. The least variation was seen in isolates from a culture with a high PsV-F level (37 units per g dry weight of mycelium). Two of the original 43 single conidial isolates contained no detectable PsV-F or PsV-S. Of the second-generation single conidial isolates from an original PsV-S PsV-F isolate, 6 % failed to show detectable PsV-S.

PsV-F levels of cultures remained constant throughout a series of transfers when the inoculum was a mixture of conidia and mycelium. The presence of PsV-F at different levels, or its complete absence, did not affect fungal biomass, conidium size, morphology or viability. The results are discussed in terms of how conidiogenesis may influence the transmission or persistence of PsV-F and PsV-S during morphogenesis.

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/content/journal/micro/10.1099/00221287-100-1-59
1977-05-01
2024-03-28
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References

  1. Adler J. P., Mackenzie D. W. 1972; Fungal viruses: intrahyphal localization of Penicilliumstoloniferum viruses by fluorescent-antibody. In Abstracts of the Annual Meeting of the American Society for Microbiology p. 68 Washington: American Society for Microbiology;
    [Google Scholar]
  2. Banks G. T., Buck K. W., Chain E. B., Himmelweit F., Marks J. E., Tyler J. M., Hollings A., Last F. T., Stone O. M. 1968; Viruses in fungi and interferon stimulation. Nature; London: 218:542–545
    [Google Scholar]
  3. Border D. J., Buck K. W., Chain E. B., Kempson-Jones G. F., Lhoas P., Ratti G. 1972; Viruses of Penicillium and Aspergillus species. Biochemical Journal 127:4P–6P
    [Google Scholar]
  4. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  5. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  6. Cole G. T., Kendrick W. B. 1969; Conidium autogeny in hyphomycetes.The phialides of Phialophora, Penicillium and Ceratocystis. Canadian Journal of Botany 47:779–789
    [Google Scholar]
  7. Detroy R. W., Lillehoj E. B., Hesseltine C. W. 1974; Replication of virus-like particles in Penicillium stoloniferum mycelia. Canadian Journal of Microbiology 20:113–117
    [Google Scholar]
  8. Ellis L. F., Kleinschmidt W. J. 1967; Virus-like particles of a fraction of statolon, a mould product. Nature; London: 215:649–650
    [Google Scholar]
  9. Fletcher J. 1971; Conidiumautogeny in Penicillium. Journal of General Microbiology 67:207–214
    [Google Scholar]
  10. Hollings M., Stone O. M. 1971; Viruses that infect fungi. Annual Review of Phytopathology 9:93–118
    [Google Scholar]
  11. Hooper G. R., Wood H. A., Myers R., Bozarth R. F. 1972; Virus-like particles in Penicillium brevicompactum and P. stoloniferum hyphae and spores. Phytopathology 62:823–825
    [Google Scholar]
  12. Lhoas P. 1971; Transmission of double stranded RNA viruses to a strain of Penicillium stoloniferum through heterokaryosis. Nature; London: 230:248–249
    [Google Scholar]
  13. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  14. Sansing G. A., Detroy R. W., Freer S. N., Hesseltine C. W. 1973; Virus particles from conidia of Penicillium species. Applied Microbiology 26:914–918
    [Google Scholar]
  15. Still P. E., Detroy R. W., Hesseltine C. W. 1975; Penicillium stoloniferum virus: altered replication in ultraviolet-derived mutants. Journal of General Virology 27:275–281
    [Google Scholar]
  16. Volterra L., Cassone A., Tonolo A., Bruzzone M. L. 1975; Presence of two virus-like particles in Penicillium citrinum. Applied Microbiology 30:149–151
    [Google Scholar]
  17. Zachariah K., Fitz-James P. C. 1967; The structure of phialides in Penicillium claviforme. Canadian Journal of Microbiology 13:249–256 (pl. I-VIII)
    [Google Scholar]
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