SUMMARY: Levels of both fast and slow Penicillium stoloniferum virus (PsV-F and PsV-S) were evaluated in single conidial isolates of P. stoloniferumnrrl5267. Approximately 7 % (3/43) of a random population of conidia from a culture of P. stoloniferum contained no PsV-F, while other conidia produced cultures with various levels of PsV-F. The greatest variation in PsV-F levels was observed in single conidial isolates from cultures that contained low to intermediate levels of virus, i.e. 11 to 25 E1cm260 units per g dry weight of mycelium. The least variation was seen in isolates from a culture with a high PsV-F level (37 E260 units per g dry weight of mycelium). Two of the original 43 single conidial isolates contained no detectable PsV-F or PsV-S. Of the second-generation single conidial isolates from an original PsV-S+ PsV-F− isolate, 6 % failed to show detectable PsV-S.
PsV-F levels of cultures remained constant throughout a series of transfers when the inoculum was a mixture of conidia and mycelium. The presence of PsV-F at different levels, or its complete absence, did not affect fungal biomass, conidium size, morphology or viability. The results are discussed in terms of how conidiogenesis may influence the transmission or persistence of PsV-F and PsV-S during morphogenesis.
AdlerJ. P.,
MackenzieD. W.1972; Fungal viruses: intrahyphal localization of Penicilliumstoloniferum viruses by fluorescent-antibody. In Abstracts of the Annual Meeting of the American Society for Microbiology p. 68 Washington: American Society for Microbiology;
BanksG. T.,
BuckK. W.,
ChainE. B.,
HimmelweitF.,
MarksJ. E.,
TylerJ. M.,
HollingsA.,
LastF. T.,
StoneO. M.1968; Viruses in fungi and interferon stimulation. Nature; London: 218:542–545
BozarthR. F.,
WoodH. A.,
MandelbrotA.1971; The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
ColeG. T.,
KendrickW. B.1969; Conidium autogeny in hyphomycetes.The phialides of Phialophora, Penicillium and Ceratocystis. Canadian Journal of Botany 47:779–789
DetroyR. W.,
LillehojE. B.,
HesseltineC. W.1974; Replication of virus-like particles in Penicillium stoloniferum mycelia. Canadian Journal of Microbiology 20:113–117
HooperG. R.,
WoodH. A.,
MyersR.,
BozarthR. F.1972; Virus-like particles in Penicillium brevicompactum and P. stoloniferum hyphae and spores. Phytopathology 62:823–825
StillP. E.,
DetroyR. W.,
HesseltineC. W.1975; Penicillium stoloniferum virus: altered replication in ultraviolet-derived mutants. Journal of General Virology 27:275–281