1887

Abstract

SUMMARY: A membrane-bound nitrate reductase from can utilize either sulphite or NADH as an electron donor. The sulphite-dependent nitrate reductase activity was released from the membrane by treatment with sodium deoxycholate. Cytochrome and FAD were separated from the solubilized enzyme by heat treatment and subsequent chromatography on DEAE-cellulose. The bacterial cytochrome and a preparation of horse-heart cytochrome served as electron mediators to the solubilized sulphite-dependent nitrate reductase activity with apparent values of 1.5 and 1.3 μM respectively. The NADH-linked enzymic activity, which was unstable during storage, was re-activated with reduced glutathione. It was also inactivated after treatment with deoxycholate but this effect was reversed by menadione. A possible scheme for electron transport for the sulphite- and NADH-dependent enzyme is proposed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-1-49
1977-05-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-1-49
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error