1887

Abstract

SUMMARY: The growth of a genital trachoma-inclusion conjunctivitis agent strain of in McCoy cells treated with cytochalasin B was studied by quantitative infectivity estimations and by light and electron microscopy. Provided that infection of the monolayer was initiated by centrifuging the infectious particles on to the cells before incubation, this chlamydial strain grew as fast and to as high a titre [approximately 10 inclusion-forming units (i.f.u.) per culture] as those chlamydiae which infect cell cultures without centrifugation. Each i.f.u. inoculated yielded approximately 600 i.f.u., and extracellular infectivity was detected soon after intracellular infectivity appeared. Inclusions were recognized by fluorescent antibody staining techniques early in the developmental cycle when cultures were not infectious and when only reticulate bodies were seen by electron microscopy. Inclusions were recognized in Giemsa-stained preparations examined by dark ground microscopy only when elementary bodies appeared in the inclusions. Iodine staining was not a reliable indicator either of the number of inclusions present or of their infectivity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-1-31
1977-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/1/mic-100-1-31.html?itemId=/content/journal/micro/10.1099/00221287-100-1-31&mimeType=html&fmt=ahah

References

  1. Armstrong J. A., Reed S. E. 1964; Nature and origin of initial bodies in lymphogranuloma venereum. Nature; London: 201:371–373
    [Google Scholar]
  2. Bedson S. P., Bland J. O. W. 1932; A morphological study of psittacosis virus with the description of a developmental cycle. British Journal of Experimental Pathology 13:461–466
    [Google Scholar]
  3. Bernkopf H., Mashiah P. 1962; The growth cycle of a trachoma agent in FL cell cultures. Journal of Immunology 88:570–571
    [Google Scholar]
  4. Bernkopf H., Mashiah P., Becker Y. 1962; Correlation between morphological and biochemical changes and the appearance of infectivity in FL cell cultures infected with trachoma agent. Annals of the New York Academy of Sciences 98:62–81
    [Google Scholar]
  5. Bland J. O. W., Canti R. G. 1935; The growth and development of psittacosis virus in tissue cultures. Journal of Pathology and Bacteriology 40:231–241
    [Google Scholar]
  6. Carter S. B. 1967; Effects of cytochalasins on mammalian cells. Nature; London: 213:261–264
    [Google Scholar]
  7. Collier L. H. 1962; Growth characteristics of inclusion blennorrhoea virus in cell cultures. Annals of the New York Academy of Sciences 98:42–49
    [Google Scholar]
  8. Evans A. 1972; The development of TRIC organisms in cell cultures during multiple infection. Journal of Hygiene 70:39–48
    [Google Scholar]
  9. Furness G., Fraser E. F. 1962; One-step growth curves for inclusion blennorrhoea virus in HeLa cell monolayers. Journal of General Microbiology 27:299–304
    [Google Scholar]
  10. Furness G., Graham D. M., Reeve P. 1960; The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. Journal of General Microbiology 23:613–619
    [Google Scholar]
  11. Gordon F. B., Harper I. A., Quan A. L., Treharne J. D., Dwyer R. ST. C., Garland J. A. 1969; Detection of Chlamydia (Bedsonia) in certain infections of man. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation. Journal of Infectious Diseases 120:451–462
    [Google Scholar]
  12. Gordon F. B., Dressler H. R., Quan A. L., Mcquilkin W. T., Thomas J. I. 1972; Effect of ionizing irradiation on susceptibility of McCoy cell cultures to Chlamydia trachomatis. Applied Microbiology 23:123–129
    [Google Scholar]
  13. Higashi N. 1964; The mode of reproduction of the psittacosis-lymphogranuloma-trachoma (PLT) group viruses. International Review of Experimental Pathology 3:35–64
    [Google Scholar]
  14. Higashi N. 1965; Electron microscopic studies on the mode of reproduction of trachoma virus (psittacosis virus) in cell cultures. Experimental and Molecular Pathology 4:24–39
    [Google Scholar]
  15. Higashi N., Tamura A., Iwanaga M. 1962; Developmental cycle and reproductive mechanism of the meningopneumonitis virus in L cells. Annals of the New York Academy of Sciences 98:100–121
    [Google Scholar]
  16. Hobson D., Johnson F. W. A., Rees E., Tait I. A. 1974; Simplified method for diagnosis of genital and ocular infections with chlamydia. Lancet ii:555–556
    [Google Scholar]
  17. Jenkin H. M. 1966; The continuous passage of agents of trachoma in cell culture. I. Characteristics of TW-3 and Bour strains of trachoma cultivated in serial passage in HeLa 229 cells. Journal of Infectious Diseases 116:390–399
    [Google Scholar]
  18. Jenkin H. M., Fan V. S. C. 1971; Contrast of glycogenesis of Chlamydia trachomatis and Chlamydia psittaci strains in HeLa cells. In Trachoma and Related Disorders pp. 52–59 Nichols R. L. Edited by Amsterdam: Excerpta Medica;
    [Google Scholar]
  19. Johnson F. W. A., Hobson D. 1976; Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of Chlamydia A (TRIC agents). Journal of Hygiene 76:441–451
    [Google Scholar]
  20. Kajima M., Sharon N., Pollard M. 1967; Virus-like particles in cultures of McCoy cells. Journal of Ultrastructure Research 18:661–666
    [Google Scholar]
  21. Kramer M. J., Gordon F. B. 1971; Ultrastructural analysis of the effects of penicillin and chloro-tetracycline on the development of a genital tract Chlamydia. Infection and Immunity 3:333–341
    [Google Scholar]
  22. Kuo C. C., Wang S. P., Wentworth B. B., Grayston J. T. 1972; Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. Journal of Infectious Diseases 125:665–668
    [Google Scholar]
  23. Litwin J., Officer J. E., Brown A., Moulder J. W. 1961; A comparative study of the growth cycles of different members of the psittacosis group in different host cells. Journal of Infectious Diseases 109:251–279
    [Google Scholar]
  24. Pollard M., Starr T. J. 1962; Study of intracellular virus with acridine orange fluorochrome. Progress in Medical Virology 4:54–69
    [Google Scholar]
  25. Rake G., Jones H. P. 1942; Studies on lymphogranuloma venereum. I. Development of the agent in the yolk sac of the chicken embryo. Journal of Experimental Medicine 75:323–338
    [Google Scholar]
  26. Richmond S. J. 1974; The isolation of Chlamydia subgroup A (Chlamydia trachomatis) in irradiated McCoy cells. Journal of Medical Laboratory Technology 31:7–9
    [Google Scholar]
  27. Richmond S. J. 1975 Chlamydia trachomatis in the human genital tract pp. 48–50 M.D. thesis Cambridge University;
    [Google Scholar]
  28. Richmond S. J. 1976; Growth of Chlamydia trachomatis in cell culture. Lancet i:865
    [Google Scholar]
  29. Richmond S. J., Caul E. O. 1975; Fluorescent antibody studies in chlamydial infections. Journal of Clinical Microbiology 1:345–352
    [Google Scholar]
  30. Ridler M. A. C., Smith G. F. 1968; The response of human cultured lymphocytes to Cytochalasin B. Journal of Cell Science 3:595–602
    [Google Scholar]
  31. Schachter J., Dawson C. R., Balas S., Jones P. 1970; Evaluation of laboratory methods for detecting acute TRIC agent infection. American Journal of Ophthalmology 70:375–380
    [Google Scholar]
  32. Sompolinsky D., Richmond S. 1974; Growth of Chlamydia trachomatis in McCoy cells treated with Cytochalasin B. Applied Microbiology 28:912–914
    [Google Scholar]
  33. Sowa J., Collier L. H., Sowa S. 1971; A comparison of the iodine and fluorescent antibody methods for staining trachoma inclusions in the conjunctiva. Journal of Hygiene 69:693–708
    [Google Scholar]
  34. Todd W. J., Storz J. 1975; Ultrastructural cytochemical evidence for the activation of lysosomes in the cytocidal effect of Chlamydia psittaci. Infection and Immunity 12:638–646
    [Google Scholar]
  35. Wang S. P., Grayston J. T. 1971; Studies on the identity of the ‘fast’ egg-killing chlamydia strains. In Trachoma and Related Disorders pp. 322–336 Nichols R. L. Edited by Amsterdam: Excerpta Medica;
    [Google Scholar]
  36. Weiss E., Dressler E. R. 1960; Centrifugation of rickettsiae and viruses onto cells and its effect on infection. Proceedings of the Society for Experimental Biology and Medicine 103691–695
    [Google Scholar]
  37. Wentworth B. B., Alexander E. R. 1974; Isolation of Chlamydia trachomatis by use of 5-iodo-2- deoxyuridine-treated cells. Applied Microbiology 27:912–916
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-1-31
Loading
/content/journal/micro/10.1099/00221287-100-1-31
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error