1887

Abstract

SUMMARY: Choline, a component of the wall teichoic acid of , was converted to cytidine diphosphocholine via choline phosphate by enzymes which were identified in cell-free extracts of the pneumococcus. The first enzyme, choline kinase, was investigated in some detail. It appeared to have a pH optimum of 7·3 to 7·4 and was stimulated by Mg. Kinetic studies gave an apparent Michaelis constant ( ) for ATP of 1 m, and for choline of 0·19 m, with values of 3 nmol min (mg protein) and 0·5 nmol min (mg protein) respectively. The second enzyme, CDPcholine pyrophosphorylase was specific for CTP and had a requirement for Mg with an optimum at 7 m.

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1977-05-01
2024-04-24
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References

  1. Bregoff H. M., Roberts E., Delwiche C. C. 1953; Paper chromatography of quaternary ammonium bases and related compounds. Journal of Biological Chemistry 205:565–574
    [Google Scholar]
  2. Briles E. B., Tomasz A. 1973; Pneumococcal Forssman antigen - a choline containing lipoteichoic acid. Journal of Biological Chemistry 248:6394–6397
    [Google Scholar]
  3. Brostrom M. A., Browning E. T. 1973; Choline kinase from Brewers’ Yeast. Journal of Biological Chemistry 248:2364–2371
    [Google Scholar]
  4. Brundish D. E., Baddiley J. 1968; Pneumococcal C-substance, a ribitolteichoic acid containing choline phosphate. Biochemical Journal 110:573–582
    [Google Scholar]
  5. Hagen P-O., Goldfine H., Williams P. J.LEB 1966; Phospholipids of bacteria with extensive intracytoplasmic membranes. Science 151:1543–1544
    [Google Scholar]
  6. Holtje J. V., Tomasz A. 1974; Teichoic acid phosphoryl-choline esterase.A novel enzyme activity in pneumococcus. Journal of Biological Chemistry 249:7032–7034
    [Google Scholar]
  7. Hunter K., Rose A. H. 1971; Yeast lipids and membranes. In The Yeasts 2 pp. 211–270 Rose A. H., Harrison J. S. Edited by London: Academic Press;
    [Google Scholar]
  8. Kennedy E. P. 1962; The metabolism and function of complex lipids. Harvey Lectures, Series 57:141–171
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Rane L., Subbarow Y. 1940; Nutritional requirements of the Pneumococcus. I. Growth factors for types 1,11, v, VII and vm. Journal of Bacteriology 40:695–704
    [Google Scholar]
  11. Riley R. F. 1944; Metabolism of phosphoryl choline.I. Synthesis of calcium phosphorylcholine chloride containing the radioactive isotope P32. Journal of the American Chemical Society 66:512–513
    [Google Scholar]
  12. Tomasz A. 1964; Studies on the competence (for genetic transformation) ofDiplococcus pneumoniae in a synthetic medium. Bacteriological Proceedings 29:29
    [Google Scholar]
  13. Tomasz A. 1967; Choline in the cell wall of a bacterium: novel type of polymer-linked choline in pneumococcus. Science 157:694–697
    [Google Scholar]
  14. Tomasz A. 1968; Biological consequences of the replacement of choline by ethanolamine in the cell wall of pneumococcus. Proceedings of the National Academy of Sciences of the United States of America 59:86–93
    [Google Scholar]
  15. Tomasz A., Westphal M., Briles E. B., Fletcher P. 1975; On the physiological functions of teichoic acids. Journal of Supramolecular Structure 3:1–16
    [Google Scholar]
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