1887

Abstract

SUMMARY: A non-helical strain of from little-leaf diseased oranges was subcultured over 40 times in different media without producing helical cells. It was non-motile and produced colonies of a characteristic morphology on soft agar plates. Serology, DNA hybridization and toxin production confirmed that it was a strain of but its membrane protein pattern after polyacrylamide gel electrophoresis differed from those of other strains in that one band was absent. The organism caused symptoms in plants identical to those produced by a pathogenic helical strain of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-100-1-15
1977-05-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/100/1/mic-100-1-15.html?itemId=/content/journal/micro/10.1099/00221287-100-1-15&mimeType=html&fmt=ahah

References

  1. Clyde W. A. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. Journal of Immunology 92:958–965
    [Google Scholar]
  2. Cole R. M., Tully J. G., Popkin T. S., Bové J. M. 1973; Morphology, ultrastructure, and bacteriophage infection of the helical mycoplasma-like organism (Spiroplasma citri gen. nov., sp. nov.) cultured from ‘Stubborn’ disease of citrus. Journal of Bacteriology 115:367–386
    [Google Scholar]
  3. Cole R. M., Tully J. G., Popkin T. J. 1974; Virus-like particles in Spiroplasma citri. In Les Mycoplasmes de l’Homme, des Animaux, des Végétaux et des Insectes INSERM International Congress pp. 125–132 Bové J. M., Duplan J. F. Edited by Paris: INSERM;
    [Google Scholar]
  4. Daniels M. J. 1969; Lipid synthesis in relation to the cell cycle of Bacillus megaterium km and Escherichia coli. Biochemical Journal 115:697–701
    [Google Scholar]
  5. Daniels M. J., Meddins B. M. 1973; Polyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate. Journal of General Microbiology 76:239–242
    [Google Scholar]
  6. Daniels M. J., Markham P. G., Meddins B. M., Plaskitt A. K., Townsend R., Bar-Joseph M. 1973; Axenic culture of a plant pathogenic spiroplasma. Nature; London: 244523–524
    [Google Scholar]
  7. Hull R. 1971; Examination of alfalfa mosaic virus protein on polyacrylamide gels. Virology 45:767–772
    [Google Scholar]
  8. Markham P. G., Townsend R., Bar-Joseph M., Daniels M. J., Plaskitt A., Meddins B. M. 1974; Spiroplasmas are the causal agent of citrus little-leaf disease. Annals of Applied Biology 75:49–57
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  10. Milne R. G., Thompson G. W., Taylor-Robinson D. 1972; Electron microscope observations on Acholeplasma laidlawii viruses. Archiv für die gesamte Virus forschung 37:378–385
    [Google Scholar]
  11. Razin S., Hasin M., Ne’Eman Z., Rottem S. 1973; Isolation, chemical composition, and ultrastructural features of the cell membrane of the mycoplasma-like organism Spiroplasma citri. Journal of Bacteriology 116:1421–1435
    [Google Scholar]
  12. Saglio P., L’Hospital M., Lafléche D., Dupont G., Bové J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp. n.: a mycoplasma-like organism associated with ‘Stubborn’ disease of citrus. International Journal of Systematic Bacteriology 23:191–204
    [Google Scholar]
  13. Saglio P., Davis R. E., Dalibart R., Dupont G., Bové J. M. 1974; Spiroplasma citri: L’espéce type des spiroplasmes. In Les Mycoplasmes de L’Homme, des Animaux, des Végétaux et des Insectes INSERM International Congress pp. 27–34 Bové J. M., Duplan J. F. Edited by Paris: INSERM;
    [Google Scholar]
  14. Scriba M. 1968; Experiments to eliminate mycoplasmas from tissue cultures by means of antibiotics. Zeitschrift für medizinische Mikrobiologie und Immunologie 154:267–276
    [Google Scholar]
  15. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; Colour test for the measurement of antibody to certain Mycoplasma species based upon acid production. Journal of Hygiene 64:91–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-100-1-15
Loading
/content/journal/micro/10.1099/00221287-100-1-15
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error