SUMMARY: When sprayed on the leaves of tobacco plants inoculated with tobacco mosaic virus 8-azaguanine caused a delay in the production of virus, and a delay in the development of systemic infection. From alkaline hydrolysates of the nucleic acid prepared from virus isolated from 8-azaguanine-treated plants, a compound was isolated which had the expected properties of 8-azaguanylic acid. This evidence together with analytical data showed that in tobacco mosaic virus from 14-day-old infections in 8-azaguanine-treated plants, about 3–4% of the guanine in the virus nucleic acid was replaced by 8-azaguanine. This incorporation of 8-azaguanine appeared to reduce the infectivity of the virus. Several other purine analogues were ineffective against tobacco mosaic virus. A serological-chromatographic method is described for the estimation of small amounts of tobacco mosaic virus in crude plant extracts.


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