1887

Abstract

Salmonella, shigella, alkalescens-dispar, proteus, paracolon and coli-form strains were tested for the presence of a coagulase enzyme as manifested by the ability to clot plasma. The shigella and proteus strains examined gave negative results; a high percentage of the remaining strains clotted citrated plasma but this reaction was due to metabolism of citrate and subsequent liberation of calcium ions and not to a coagulase enzyme. Results depended on the bacterial strain and on the kind and dilution of plasma used. Utilization of citrate in citrated plasma was compared with the ability of the tested strains to attack citrate in Koser’s ammonium citrate medium and in sodium citrate peptone medium. The and species examined failed to utilize citrate in any of the media under the conditions of the tests, species gave fairly uniform results in the three media, while paracolon, coliform and alkalescens-dispar strains differed in their activity, the highest percentage of positive reactions being obtained in plasma-containing media.

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1954-06-01
2021-10-23
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References

  1. Bergey’s Manual of Determination Bacteriology 1948, 6th ed. Breed R. S., Murray E. G. D., Hitchens A. P. Edited by Baltimore: The Williams & Wilkins Co;
    [Google Scholar]
  2. Cook G. T. 1948; Urease and other biochemical reactions of the Proteus group. J. Path. Bact 60:171
    [Google Scholar]
  3. Corda M. 1947; Sulla coagulazione del sangue citratato ad opera della S. typhi. Boll. Soc. ital. Biol. sper 23:842
    [Google Scholar]
  4. Evans J. B., Buettner L. G., Niven C. F. Jun 1952; Occurrence of streptococci that give a false positive coagulase test. J. Bact 64:433
    [Google Scholar]
  5. Fisher A. M. 1936; The plasma coagulating properties of staphylococci. Johns Hopk. Hosp. Bull 59:393
    [Google Scholar]
  6. Fredericq P. 1942; La coagulation du sang en l’absence de calcium. Arch. int. Physiol 42:73
    [Google Scholar]
  7. Fredericq P. 1946a; Sur la coagulation du plasma oxalaté par les cultures de B. prodigiosus. C.R. Soc. Biol., Paris 140:1132
    [Google Scholar]
  8. Fredericq P. 1946b; Sur la coagulation du plasma oxalaté par les cultures d’Actinomyces. C.R. Soc. Biol., Paris 140:1166
    [Google Scholar]
  9. Gross H. 1931; Der Plasmagerinnungsstoff der Staphylokokken. Zbl. Bakt. (1.Abt. Orig.) 122:354
    [Google Scholar]
  10. Harper E. M., Conway N. S. 1948; Clotting of human citrated plasma by Gram-negative organisms. J. Path. Bact 60:247
    [Google Scholar]
  11. Kauffmann F. 1951 Enterobaderiaceae Copenhagen: Ejnar Munksgaard;
    [Google Scholar]
  12. Kemkes B. 1928; Plasmakoagulase und Pathogenität der Staphylokokken. Zbl. Bakt. (1.Abt. Orig.) 109:11
    [Google Scholar]
  13. Koser S. A. 1924; Correlation of citrate utilization by members of the colon-aerogenes group with other differential characteristics and with habitat. J. Bact 9:59
    [Google Scholar]
  14. Krech U. 1952; Untersuchungen über die Koagulation von menschlichem und tierischem Plasma durch Bacterium coli. Z. ImmunForsch 109:206
    [Google Scholar]
  15. Loeb L. 1903; The influence of certain bacteria on the coagulation of the blood. J. med. Res 10:407
    [Google Scholar]
  16. Lominski I., Conway N. S., Harper E. M., Rennie J. B. 1947; Utilization of citric acid by some so-called citrate-non-utilizing bacteria. Nature; Lond: 160573
    [Google Scholar]
  17. Lusena M. 1929; Fermenti coagulanti di origine batterica. Boll. 1st. sieroter. Milano 8:559 Abst. in Zbl. Bakt. (1.Abt. Ref.), 1930, 99: 179
    [Google Scholar]
  18. Much H. 1908; Über eine Vorstufe des Fibrinfermentes in Kulturen von Staphylo- kokkus aureus. Biochem. Z 14:143
    [Google Scholar]
  19. Reimer K. von. 1936; Über charakteristische Unterschiede in der gerinnungs-beeinflussenden und fibrinauflösenden Wirkung lebender Bakterien. Zbl. Bakt. (1. Abt. Orig.) 136:84
    [Google Scholar]
  20. Rita G. 1945a; Richerche sulla coagulazione del plasma salato ad opera di alcuni ceppi di B. typhi. Boll. Soc. ital. Biol. sper 20:224
    [Google Scholar]
  21. Rita G. 1945b; Sulla natura del fenomeno della coagulazione del plasma citratato ad opera di alcuni ceppi di B. typhi. Boll. Soc. ital. Biol. sper 20:227
    [Google Scholar]
  22. Rita G. 1945c; Sulla coagulazione del plasma citratato ad opera dei BB. paratifici e di altre Salmonelle. Boll. Soc. ital. Biol. sper 20:351
    [Google Scholar]
  23. Rustigan R., Stuart C. A. 1945; The biochemical and serological relationships of the organisms of the genus Proteus. J. Bact 49:419
    [Google Scholar]
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