1887

Abstract

SUMMARY: Strains of belonging to Types 2 and 4 elaborated an amylolytic enzyme which was not usually a product of other serological Types though it was occasionally produced by members of Types 1, 9, 22 and Types related to 4, 5 and 25. Sixty-six % of amylolytic strains were untypable by M precipitin tests.

The enzyme was produced by variants. Amylolytic organisms were generated by matt and large opaque colonies of the parent strains; small and glossy variants generated relatively fewer enzyme-positive cocci, and had often lost the amylolytic property. When studied as a single character in serial subcultures on starch agar, the property was transmissible, in some instances to 80 % of the progeny for many generations (forty or more subcultures). Other strains readily lost the property in subculture on starch agar but recovered amylolytic power during passage on plasma agar in the absence of substrate. Certain amylolytic strains contained a trypsin-resistant, serologically active substance which precipitated in anti-M, anti-T and anti-proteinase precursor serum. A similar substance diffused into the medium when strains were grown on agar containing the substrate. While serologically related to the cell antigen, the free substance was trypsin-sensitive.

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1954-06-01
2021-10-27
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References

  1. Crowley N. 1944; Hyaluronidase production by haemolytic streptococci of human origin. J. Path. Bact 56:27
    [Google Scholar]
  2. Crowley N. 1950; The degradation of starch by Group A streptococci having related antigens. J. gen. Microbiol 4:156
    [Google Scholar]
  3. Elliott S. D. 1945; A proteolytic enzyme produced by Group A streptococci with special reference to its effect on the type specific M antigen. J. exp. Med 81:573
    [Google Scholar]
  4. Elliott S. D. 1950; The crystallisation and serological differentiation of a streptococcal proteinase and its precursor. J. exp. Med 92:201
    [Google Scholar]
  5. Elliott S. D., Dole V. P. 1947; An inactive precursor of streptococcal proteinase. J. exp. Med 85:305
    [Google Scholar]
  6. Griffith F. 1934; The serological classification of Streptococcus pyogenes. J. Hyg., Camb 34:542
    [Google Scholar]
  7. Lancefield R. C. 1940; Type-specific antigens M and T, or matt and glossy variants of Group A streptococci. J. exp. Med 71:539
    [Google Scholar]
  8. Lancefield R. C., Dole V. P. 1946; The properties of T antigens extracted from Group A streptococci. J. exp. Med 84:449
    [Google Scholar]
  9. Lancefield R. C., Peblman G. E. 1952; Preparation and properties of a protein (R antigen) occurring in streptococci of Group A, Type 28 and in certain streptococci of other serological groups. J. exp. Med 96:83
    [Google Scholar]
  10. Maxted W. R. 1948; Preparation of streptococcal extracts for Lancefield grouping. Lancet ii:255
    [Google Scholar]
  11. Maxted W. R. 1953; The M and T antigens of streptococcus pyogenes Type 2. J. Path. Bact 65:345
    [Google Scholar]
  12. McClean D. 1942; The in vivo decapsulation of streptococci by hyaluronidase. J. Path. Bact 54:284
    [Google Scholar]
  13. Olarte J. 1948; Demonstration of the ‘M’ protein in culture filtrates of haemolytic streptococci of Group A. J. Immunol 58:15
    [Google Scholar]
  14. Pakula R. 1951; Extraction of the T antigen of Streptococcus pyogenes. J. gen. Microbiol 5:640
    [Google Scholar]
  15. Stanier R. Y. 1953; Adaptation, evolutionary and physiological; or Darwinism among the micro-organisms. Symp. Soc. gen. Microbiol 3:1
    [Google Scholar]
  16. Stewart W. A., Swift H., Wilson A., Lancefield R. C. 1944; Studies on the antigenic composition of Group A haemolytic streptococci. IV.Related T but distinct M antigens in Types 15, 17, 19, 23, 30 and in Types 4, 24, 26, 28, 29, 46. Identification by slide agglutination. J. exp. Med 79:99
    [Google Scholar]
  17. Swift H., Wilson A., Lancefield R. C. 1943; The typing of Group A streptococci by M precipitin reactions in capillary pipettes. J. exp. Med 78:127
    [Google Scholar]
  18. Todd E. W., Hewitt L. 1932; A new culture medium for the production of antigenic haemolysin. J. Path. Bact 95:873
    [Google Scholar]
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