1887

Abstract

The cell wall of a bacillus, related to , reacts specifically with the homologous antibody. This reaction can be made clearly visible by phase contrast when cell wall and cytoplasmic membrane are separated by mild lysozyme digestion, or when the cytoplasm is partially autolysed or digested with trypsin. The same antibody reacts with the transverse septa and polar bodies of the capsule, rendering them visible by phase contrast. These septa are in continuation of the cross walls of the cell, and sometimes give the appearance of division.

By these means the morphological locations of the chemically isolable fractions of serologically active bacterial extracts can be defined. These substances in the cell wall and in the septa and polar bodies of the capsule appear to be polysaccharide. The -glutamic acid polypeptide element of the capsule does not occur in the cell wall.

A non-specific cell-wall reaction can be obtained with proteins at the appropriate values of pH, analogous to the non-specific capsular reaction previously reported.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-10-2-317
1954-04-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/10/2/mic-10-2-317.html?itemId=/content/journal/micro/10.1099/00221287-10-2-317&mimeType=html&fmt=ahah

References

  1. Bisset K. A. 1948; The cytology of smooth and rough variation in bacteria. J. gen. Microbiol 2:83
    [Google Scholar]
  2. Bisset K. A. 1950 The Cytology and Life-history of Bacteria Edinburgh: E. and S. Livingstone, Ltd;
    [Google Scholar]
  3. Gladstone G. P., Fildes P. 1940; A simple culture medium for general use without meat extract or peptone. Brit. J. exp. Path 21:161
    [Google Scholar]
  4. Knaysi G. 1951 Elements of Bacterial Cytology, 2nd ed. Ithaca, N.Y: Comstock;
    [Google Scholar]
  5. Mudd S., Anderson T. F. 1941; Demonstration by the electron microscope of the combination of antibodies with flagellar and somatic antigens. J. Immunol 42:251
    [Google Scholar]
  6. Neufeld F. 1902; Über die Agglutination der Pneumokokken und über die Theorien der Agglutination. Z. Hyg. InfectKr 40:54
    [Google Scholar]
  7. Tomcsik J. 1950; Über eine bewegliche Mutante des Bacillus anthracis. Schweiz. Z. allg. Path 13:616
    [Google Scholar]
  8. Tomcsik J. 1951; Complex structure of the bacterial capsule in the genus Bacillus. Experientia 7:459
    [Google Scholar]
  9. Tomcsik J., Guex-Holzer S. 1951; Anthrax-Polpeptid und andere spezies-spezifische Substanzen der Kapsel in der Bazillus-Gruppe. Schweiz. Z. allg. Path 14:515
    [Google Scholar]
  10. Tomcsik J., Guex-Holzer S. 1952; Änderung der Struktur der Bakterienzelle im Verlauf der Lysozym-Einwirkung. Schweiz. Z. allg. Path 15:518
    [Google Scholar]
  11. Tomcsik J., Guex-Holzer S. 1953; Ein neues Prinzip zur Färbung der Bakterienkapsel. Schweiz. Z. allg. Path 16:882
    [Google Scholar]
  12. Tomcsik J., Guex-Holzer S. 1954; Demonstration of the bacterial capsule by means of a pH-dependent, salt-like combination with proteins. J. gen. Microbiol 10:97
    [Google Scholar]
  13. Welshimer H. J., Robinow C. F. 1949; The lysis of Bacillus megatherium by lysozyme. J. Bact 57:489
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-10-2-317
Loading
/content/journal/micro/10.1099/00221287-10-2-317
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error