%0 Journal Article %A Few, A. V. %T Electron Microscopy of Disrupted Bacteria treated with Polymyxin E %D 1954 %J Microbiology, %V 10 %N 2 %P 304-308 %@ 1465-2080 %R https://doi.org/10.1099/00221287-10-2-304 %I Microbiology Society, %X SUMMARY: Mechanically disrupted cells of polymyxin E sensitive strains of Pseudomonas denitrificans and Escherichia coli and a resistant strain of Staphylococcus aureus were studied in the electron microscope. The occurrence of a secondary layer or membrane was observed within the outer cell walls derived from Ps. denitrificans and E. coli; a similar structure was not observed in cell walls prepared from Staph. aureus. Treatment of the disrupted cells of Ps. denitrificans and E. coli with polymyxin E resulted in marked morphological changes, whilst similar exposure of Staph. aureus cell walls to the antibiotic produced no significant changes in their morphology. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-10-2-304