SUMMARY: The production by certain species of of enzymes disintegrating hide powder was investigated by measuring the lytic action of broth cultures and toxic filtrates on finely divided hide powder suspended in an agar gel.

was the most active producer of enzyme, A was less active and and only moderately active. and produced no such enzyme. The lytic enzyme of is not the lethal toxin.

Among strains of type A, enzyme production, α-toxin production and ability to cause fatal infection in guinea-pigs are associated.

There was some evidence that the different enzymes affecting hide powder are antigenically related, but no definite conclusion is possible, since the antisera employed may have contained antibodies to the lytic enzymes of a number of different organisms.


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