Spray Inoculation of Plates in the Detection of Antagonistic Micro-organisms

SUMMARY: By means of a simple atomizer the surface of agar media may be sprayed with a suspension of micro-organisms, so that an even growth is obtained. The method is suitable for the seeding of plates for the assay of antimicrobial substances. It is particularly valuable for the detection of microbial antagonisms, for a test organism may be seeded on to established plate cultures of other organisms, without disturbing the bacterial colonies already present on the agar surface.