RT Journal Article SR Electronic(1) A1 Ainsworth, G. C. A1 Brown, Annie M. A1 Marsden, P. S. S. F. A1 Smith, P. A. A1 Spilsbury, J. F.YR 1947 T1 A Method for the Large-scale Production of Streptomycin by Surface Culture JF Microbiology, VO 1 IS 3 SP 335 OP 343 DO https://doi.org/10.1099/00221287-1-3-335 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Working details are given of a method for producing streptomycin by the surface culture of Streptomyces griseus in pint milk bottles on a papain digest of beef+meat extract+glucose+mineral salt medium. Streptomycin titres in the crude culture filtrates of 250 µg./ml. or more were obtained after 10–14 days’ growth. Evans’s peptone, papain digest of spent pancreas from insulin manufacture, papain digest of yeast, and a proprietary casein-meat hydrolysate were found to be possible alternative sources of organic nitrogen. Growth was good on media containing peptic digest of beef or peptic-tryptic casein digest, but the streptomycin there was low. The utilization of glucose and the production of streptomycin depended on the relative amount of nitrogen present in the medium., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-1-3-335