@article{mbs:/content/journal/micro/10.1099/00221287-1-3-335, author = "Ainsworth, G. C. and Brown, Annie M. and Marsden, P. S. S. F. and Smith, P. A. and Spilsbury, J. F.", title = "A Method for the Large-scale Production of Streptomycin by Surface Culture", journal= "Microbiology", year = "1947", volume = "1", number = "3", pages = "335-343", doi = "https://doi.org/10.1099/00221287-1-3-335", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-1-3-335", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Working details are given of a method for producing streptomycin by the surface culture of Streptomyces griseus in pint milk bottles on a papain digest of beef+meat extract+glucose+mineral salt medium. Streptomycin titres in the crude culture filtrates of 250 µg./ml. or more were obtained after 10–14 days’ growth. Evans’s peptone, papain digest of spent pancreas from insulin manufacture, papain digest of yeast, and a proprietary casein-meat hydrolysate were found to be possible alternative sources of organic nitrogen. Growth was good on media containing peptic digest of beef or peptic-tryptic casein digest, but the streptomycin there was low. The utilization of glucose and the production of streptomycin depended on the relative amount of nitrogen present in the medium.", }