1887

Abstract

SUMMARY: The effect on glutamic acid assimilation of the addition of penicillin to growing cultures of is described. When is grown in media containing glutamic acid this substance accumulates in steadily increasing concentration in the cells. The addition of penicillin to the medium is followed after an interval by rapidly decreasing concentration of glutamic acid within the cells.

The assimilation of glutamic acid by normal washed cells is not affected by penicillin in high concentration. The assimilation of glutamic acid by cells which have grown in the presence of penicillin is impaired and may be completely inhibited. Complete inhibition of assimilation is brought about by bactericidal concentrations of penicillin, low concentrations requiring a longer time to become completely effective than high ones. The loss of assimilatory power can be correlated with loss of viability.

Comparison of the general properties of normal and penicillin-inactivated cells show that the respiration, glucose oxidation, glucose fermentation and lysine assimilation of the latter are normal. The internal metabolism of glutamic acid is normal in penicillin-treated cells, but, since the passage of glutamic acid across the cell wall is blocked, is limited by the existing internal concentration.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-1-3-314
1947-09-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/1/3/mic-1-3-314.html?itemId=/content/journal/micro/10.1099/00221287-1-3-314&mimeType=html&fmt=ahah

References

  1. Abraham E. P., Chain E., Fletcher C. M., Florey H. W., Gardner A. D., Heatley N. G., Jennings M. A. 1941; Further observations on penicillin. Lancet ii:177
    [Google Scholar]
  2. Atkinson N., Stanley N. F. 1943; Antibacterial substances produced by moulds. 4. The detection and occurrence of suppressors of penicidin. 5. The mechanism of the action of some penicidin suppressors. Aust. J. exp. Biol. med. Set 21:248–255
    [Google Scholar]
  3. Cavillito C. J., Bailey J. H., Haskell T. H., Mccormick J. R., Warner W. H. 1945; The inactivation of antibacterial agents and their mechanism of action. J. Bact 50:61
    [Google Scholar]
  4. Chain E., Duthie E. S. 1945; Bactericidal and bacteriolytic action of penicillin on staphylococcus. Lancet i:652
    [Google Scholar]
  5. Chow B. F., Mckee C. M. 1945; .Inactivation of the antibiotic action of penicillin by cysteine-hydrochloride. I. Chemical aspects of inactivation. Proc. Soc. exp. Biol., N.Y 58:175
    [Google Scholar]
  6. Fleming A. 1929; On the antibacterial action of cultures of a Penicillium, with special reference to their use in the isolation of B. influenzae. Brit. J. exp. Path 10: 226
    [Google Scholar]
  7. Gale E. F. 1947a; The assimilation of amino-acids by bacteria. 1. The passage of certain amino-acids across the cell wall and their concentration in the internal environment of Streptococcus faecalis. J. gen. Microbiol 1:53
    [Google Scholar]
  8. Gale E. F. 1947b; The assimilation of amino-acids by bacteria. 6. The effect of protein synthesis on glutamic acid accumulation and the action thereon of sulphathiazole. J. gen. Microbiol 1:327
    [Google Scholar]
  9. Gale E. F., Mitchell P. D. 1947; The assimilation of amino-acids by bacteria. 4. The action of triphenylmethane dyes on glutamic acid assimilation. J. gen. Microbiol 1:299
    [Google Scholar]
  10. Gale E. F., Taylor E. S. 1946; Action of penicillin in preventing the assimilation of glutamic acid by Staphylococcus aureus. Nature; Lond: 158676
    [Google Scholar]
  11. Gale E. F., Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 2. The action of tyrocidin and some detergent substances in releasing amino-acids from the internal environment of Streptococcus faecalis. J. gen. Microbiol 1:77
    [Google Scholar]
  12. Gardner A. D. 1940; Morphological effects of penicillin on bacteria. Nature; Lond: 146837
    [Google Scholar]
  13. Hirsch J. 1943–4; Penicillin-Studien in vitro Über den Wirkungsmodus des Penicillins. C. R. Ann. Arch. Soc. Turque Sci. Phys. Nat. Fasc 12:
    [Google Scholar]
  14. Hobby G. L., Dawson M. H. 1944; Bacteriostatic action of penicillin on hemolytic streptococci in vitro. Effect of rate of growth of bacteria on action of penicillin. Proc. Soc. exp. Biol., N.Y 56:178–181
    [Google Scholar]
  15. Hobby G. L., Meyer K., Chaffee E. 1942; Activity of penicillin in vitro. Observations on the mechanism of action of penicillin. Proc. Soc. exp. Biol., N.Y 50:277–281
    [Google Scholar]
  16. Krampitz L. O., Werkman C. H. 1947; On the mode of action of penicillin. Arch. Biochem 12:57
    [Google Scholar]
  17. Shwartzman G. 1946; Studies on the nature of resistance of Gram-negative bacilli to penicillin: antagonistic and enhancing effects of amino-acids. J. exp. Med 83:65
    [Google Scholar]
  18. Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 3. Concentration of free amino-acids in the internal environment of various bacteria and yeasts. J. gen. Microbiol 1:86
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-1-3-314
Loading
/content/journal/micro/10.1099/00221287-1-3-314
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error